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Sample GSM1347955 Query DataSets for GSM1347955
Status Public on Sep 01, 2014
Title PyMT Tumor A
Sample type RNA
Source name mouse mammary tumor
Organism Mus musculus
Characteristics strain: FVB/N
genotype: PyMT
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol (Invitrogen) and the RNEasy mini kit (Qiagen) with on-column DNAse digestion was used following Trizol extraction. 100ng total RNA per sample was used for array analysis.
Label biotin
Label protocol Fragmented ssDNA was labeled with the Affymetrix WT Terminal Labeling and Hybridization kit according to the instructions, using Affymetrix proprietary biotinylated labeling reagent.
Hybridization protocol Samples were hybridized using the Gene Atlas Microarray System (Affymetrix) hybridization chamber; 20 hours at 48 C
Scan protocol Samples were scanned and imaged using the Gene Atlas Microarray System Fludics and Imaging equipment (Affymetrix)
Data processing CEL files were processed in Partek Genomic Suite using RMA Quantile normalization; intensity values were scaled by log2 transformation
Submission date Mar 13, 2014
Last update date Sep 01, 2014
Contact name Elizabeth A Wellberg
Organization name University of Oklahoma HSC
Department Pathology
Street address 975 NE 10th St, BRC 309
City Oklahoma City
State/province Oklahoma
ZIP/Postal code 73104
Country USA
Platform ID GPL11533
Series (1)
GSE55886 Effect of Spot14 Loss on Gene Expression Profile of MMTV-PyMT Mouse Tumors

Data table header descriptions
VALUE RMA-normalized, log2 transformed intensity values

Data table
10338001 10.5043
10338002 4.86128
10338003 8.92036
10338004 7.88834
10338005 2.58223
10338006 2.76805
10338007 2.94051
10338008 3.28788
10338009 6.21348
10338010 2.58994
10338011 4.38301
10338012 2.65166
10338013 2.45811
10338014 2.52601
10338015 2.46521
10338016 5.79906
10338017 11.0879
10338018 5.19527
10338019 4.03406
10338020 6.17447

Total number of rows: 35556

Table truncated, full table size 586 Kbytes.

Supplementary file Size Download File type/resource 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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