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Sample GSM1347708 Query DataSets for GSM1347708
Status Public on Jul 29, 2014
Title MSC donor2_1.5 kPa
Sample type RNA
Source name mesenchymal stromal cells (MSC)
Organism Homo sapiens
Characteristics cell type: MSC
substrate: 1.5 kPa PDMS
donor: donor2
Treatment protocol MSCs were cultivated for 4 passages in parrallel on TCP, 50 kPa PDMS and 1.5 kPa PDMS.
Growth protocol MSCs were cultivated in 10% hPL-Medium at 37°C at humified atmosphere containing 5% carbon dioxide with medium changes twice per week. Passaging was carried out upon 80% confluent growth. MSCs were isolated from lipoaspirates after patient’s written consent using guidelines approved by the Ethic committee of the RWTH Aachen University Medical School (Permit Number EK163/07).
Extracted molecule total RNA
Extraction protocol RNA was isolated from 106 cells using the NucleoSpin RNA Isolation Kit.
Label biotin
Label protocol Standard Affymetrix protocol.
Hybridization protocol Standard Affymetrix protocol.
Scan protocol Arrays were scanned using Affymetrix GeneChip Scanner 3000.
Description gene expression data of MSC donor2 cultivated for 4 passages on 1.5 kPa PDMS
Data processing CEL files were processed using the Affymetrix Power Tools (APT) Software Package. Normalization was done employing RMA (Irizarry et al., Biostatistics 4, 249-264, 2003).
Submission date Mar 13, 2014
Last update date Jul 29, 2014
Contact name Wolfgang Wagner
Phone +49 241 8088611
Organization name RWTH Aachen University
Department Helmholtz Institute for Biomedical Engineering
Lab Stem Cell Biology and Cellular Engineering
Street address Pauwelsstrasse 20
City Aachen
ZIP/Postal code 52074
Country Germany
Platform ID GPL10739
Series (2)
GSE55867 Matrix Elasticity Does Not Affect Replicative Senescence or DNA Methylation Patterns of Mesenchymal Stem Cells [gene expression profiling]
GSE55889 Matrix Elasticity Does Not Affect Replicative Senescence or DNA Methylation Patterns of Mesenchymal Stem Cells

Data table header descriptions
VALUE RMA signal

Data table
7896739 3.40881
7896741 3.75342
7896743 7.81104
7896745 5.58350
7896751 5.86225
7896753 10.42058
7896755 8.71701
7896757 7.51633
7896758 8.33073
7896762 10.86540
7896763 6.75469
7896764 8.06298
7896766 7.64432
7896768 11.06591
7896771 8.27961
7896773 8.58923
7896776 8.30948
7896777 8.04781
7896778 7.19085
7896783 8.87501

Total number of rows: 190269

Table truncated, full table size 3022 Kbytes.

Supplementary file Size Download File type/resource
GSM1347708_AT59_1_5_kPa_HuGene-1_0-st-v1_.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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