|
| Status |
Public on Jul 31, 2014 |
| Title |
Saliva01 |
| Sample type |
genomic |
| |
|
| Source name |
Saliva
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: Saliva
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA (1 µg) from each sample was bisulfite converted using the EZ DNA methylation kit (Zymo Research), and 200 ng of the converted DNA was used for amplification.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
standard Illumina protocol
|
| |
|
| Hybridization protocol |
Amplified DNA was hybridized on the Infinium HumanMethylation 450 BeadChip (Illumina), following the Illumina Infinium HD Methylation protocol.
|
| Scan protocol |
The arrays were imaged using the Illumina HiScan SQ scanner, and image processing and extraction of intensity data were performed according to Illumina’s instructions.
|
| Description |
Saliva
Sample7
|
| Data processing |
Each methylation signal was used to compute the β value, which is a quantitative measure of DNA methylation ranging from 0 (for completely unmethylated cytosines) to 1 (for completely methylated cytosines) (Bibikova et al., 2006).
|
| |
|
| Submission date |
Mar 10, 2014 |
| Last update date |
Jul 31, 2014 |
| Contact name |
Jonglyul Park |
| E-mail(s) |
nlcguard@kribb.re.kr
|
| Organization name |
Korea Research Institute of Bioscience and Biotechnology
|
| Department |
Medical genomics
|
| Street address |
52, Eoeun-dong, Yuseong-gu
|
| City |
daejeon |
| ZIP/Postal code |
305-806 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE55734 |
Genome-wide profiling and validation for forensic tissue-specific DNA methylation markers |
|
