|
| Status |
Public on Mar 09, 2014 |
| Title |
genomic DNA from patient mHGA11 |
| Sample type |
genomic |
| |
|
| Source name |
mHGA11
|
| Organism |
Homo sapiens |
| Characteristics |
mutation: H3F3A.K27M
tissue: glioblastomas
brain region: Supratentorial Midline (GBM)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted and purified from LBC samples using Qiagen DNeasy Kit according to standard instructions
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450k Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Data processing |
We removed probes with missing values, binding multiple genomic regions, binding targets with known sequence variants (1000 Genomes Project, SNPs with a minor allele frequency ≥ 2/120), or being located on the X or Y chromosomes. The final set contains 309,015 probes
|
| |
|
| Submission date |
Mar 08, 2014 |
| Last update date |
Jan 05, 2015 |
| Contact name |
Simon Papillon-Cavanagh |
| Organization name |
McGill University
|
| Department |
Human Genetics
|
| Lab |
Majewski
|
| Street address |
740 Dr Penfield
|
| City |
Montrea |
| State/province |
Quebec |
| ZIP/Postal code |
H3A0G1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE55712 |
Genome wide methylation profliling of pediatric glioblastomas. |
|
