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Sample GSM1339127 Query DataSets for GSM1339127
Status Public on Jul 23, 2015
Title WT primary myoblasts, biological replicate 4
Sample type RNA
Source name 189
Organism Mus musculus
Characteristics genotype: wt
cell type: Myobalst
genetic background: C57bl/6
Treatment protocol comparison of WT to KO PM
Growth protocol We isolatedf SC from gastrocnemius muscles of 2-3 weeks old C57bl/6 WT or Runx1f/f mice. Cells were grown at 37°C, 5% CO2 on GHR MatrigelTM (BD Biosciences, USA) in proliferation medium (BIOAMF-2, Biological Industries, Israel), which was replaced daily
Extracted molecule total RNA
Extraction protocol Total RNA was isolated by PerfectPure RNA tissue kit (# 2302410, 5 PRIME, Germany) according to the manufacturer's instructions.
Label biotin
Label protocol 200ng TotalRNA were used for labeling. cDNA samples were generated and labeled using Affymetrix WT-cDNA synthesis and labeling kit
Hybridization protocol 2ug ss cDNA in 80 ul total volume were hybridized to a Mouse Gene 1.0 ST array using Affymetrix hybridization kit materials and hybridized for 17 hours at 45° at 60rpm. Post-hyb washes were done with Affymetrix wash solutions on the FS450 station using FS450_0007 protocol.
Scan protocol Arrays were scanned on the Affymetrix GeneChip Scanner 3000 7G
Data processing The analysis was carried out using Partek Genome Suite software (Partek Inc., St. Louis, Missouri 63141,, using RMA.
Submission date Mar 04, 2014
Last update date Jul 23, 2015
Contact name kfir baruch Umansky
Phone 97289342318
Organization name Weizmann Institute
Street address POB 25
City Rehovot
State/province Rehovot
ZIP/Postal code 76100
Country Israel
Platform ID GPL6246
Series (2)
GSE55556 Transcription factor Runx1 cooperates with MyoD and c-Jun to regulate the balance of myoblast proliferation/differentiation [Expression Study]
GSE56131 Transcription factor Runx1 cooperates with MyoD and c-Jun to regulate the balance of myoblast proliferation/differentiation

Data table header descriptions

Data table
10338068 3.40118
10338069 4.12107
10338070 4.02603
10338071 4.33121
10338072 3.31006
10338073 4.13084
10338074 8.83685
10338075 7.01728
10338076 4.95342
10338077 9.8846
10338078 3.44042
10338079 3.61723
10338080 4.17132
10338081 3.20831
10338082 5.31753
10338083 3.78402
10338084 5.65471
10338085 9.85852
10338086 4.30619
10338087 7.26394

Total number of rows: 35399

Table truncated, full table size 583 Kbytes.

Supplementary file Size Download File type/resource
GSM1339127_YG309_MoST.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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