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Sample GSM1333951 Query DataSets for GSM1333951
Status Public on Mar 25, 2014
Title E11.5 mouse lung
Sample type RNA
 
Source name mouse lung E11.5
Organism Mus musculus
Characteristics tissue: lung
stain: ICR
Treatment protocol Male and female mice were mated and the day of identification of a vaginal plug was E0.5. After 11days, mouse embryos were collected as E11.5.
Growth protocol ICR mouse were harbored following the guidelines of the National Institute of Advanced Industrial Science and Technology Animal Experiment Committee.
Extracted molecule total RNA
Extraction protocol The primordium stomach was dissected from mouse embryo at E11.5. Total RNA was isolated from stomach using the ‘RNeasy micro kit’ (Quiagen) following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer.
Label Cy3
Label protocol RNA was labeled using the ‘Agilent Low Input Quick Amp Kit’ (Agilent Technologies) following the manufacturer’s protocol. Cy3-labeled cRNA was purified with the RNeasy kit (Qiagen). Dye incorporation was assessed with the NanoDrop-1000 Spectrophotometer.
 
Hybridization protocol 1.65 ug of Cy3-labelled cRNA was fragmented at 60°C for 30 minutes in a reaction volume of 55 ul containing 25x Agilent fragmentation buffer and 10x Agilent blocking agent following the manufacturers instructions. On completion of the fragmentation reaction, 55 ul of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent Whole Mouse Genome Oligo Microarrays (G4122F) for 18 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent), 1 minute with 37°C GE Wash buffer 2 (Agilent), and 30 second at room temperature with acetonitrile, then dried immediately.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting for 1x44k array slides.
Data processing The scanned images were analyzed with Feature Extraction Software 10. 5 (Agilent) using default parameters (protocol GE1_105_Dec08 and Grid: 014868_D_20070820) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non positive and significant, and Feature Non above background outliers were excluded.
 
Submission date Feb 25, 2014
Last update date Mar 25, 2014
Contact name Mari Sekine
E-mail(s) s1220999@u.tsukuba.ac.jp
Organization name The University of Tsukuba
Department Graduate School of Life and Environmental Sciences
Street address 1-1-1 Tennoudai
City Tsukuba
ZIP/Postal code 305-8577
Country Japan
 
Platform ID GPL7202
Series (1)
GSE55312 Transcription profile of primordium gut organs

Data table header descriptions
ID_REF
VALUE Normalized log2 signal intensity

Data table
ID_REF VALUE
A_52_P616356 -2.3393135
A_52_P580582 -1.508949
A_52_P403405 -2.4202342
A_52_P819156 -1.8843608
A_51_P331831 1.2638407
A_51_P430630 -2.7157004
A_52_P502357 -2.2431166
A_52_P299964 -2.1053815
A_51_P356389 -1.9481609
A_52_P684402 -1.4207432
A_51_P414208 -3.9210663
A_51_P280918 1.2864251
A_52_P613688 -1.7808757
A_52_P258194 -1.3983145
A_52_P229271 -2.489347
A_52_P214630 2.0434527
A_52_P579519 -1.2118268
A_52_P979997 -2.5349224
A_52_P453864 -3.1630216
A_52_P655842 -1.7446246

Total number of rows: 41265

Table truncated, full table size 944 Kbytes.




Supplementary file Size Download File type/resource
GSM1333951_1_e11.5_lun.txt.gz 2.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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