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Sample GSM1332748 Query DataSets for GSM1332748
Status Public on Feb 22, 2014
Title appendiceal_orifice_patient23
Sample type RNA
Source name Appendiceal orifice
Organism Homo sapiens
Characteristics tissue: Intestinal mucosa
gender: Male
age: 60
Treatment protocol N/A
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol Pinch biopsy that was snap frozen in Trizol for RNA extraction using chloroform purification and isopropanol extraction and purification using QIAgen Rneasy Mini Kit
Label Cy3
Label protocol Labeling was performed with Cy3 according to the manufacturer protoocl for one color microarray based gene expression analysis (Agilent)
Hybridization protocol Cy3 labeled, linearly amplified complementary RNA was synthesized and hybridized to Sureprint G3 Human Gene Expression 8 x 60K (Agilent) microarrays according to manufacturer's protocol.
Scan protocol Arrays were scanned using a High Resolution C scanner
Data processing Raw signal intensities were extracted with feature extraction v10.7 software with the protocol GE1_107_Sep09 and Grid 028004_D_F_20110819, to obtain spatially detrended signal intensities.
Submission date Feb 21, 2014
Last update date Feb 22, 2014
Contact name Mei San Tang
Organization name Washington University in St Louis
Department Pathology & Immunology
Street address 660 S Euclid, Campus Box 8118
City Saint Louis
State/province MO
ZIP/Postal code 63108
Country USA
Platform ID GPL13607
Series (1)
GSE55258 Th17, Th22 and Treg cells are enriched in the healthy human cecum

Data table header descriptions
VALUE Expression intensities were log2-transformed, quantile normalized and median-centered.

Data table
4 0.040508671
5 -0.090798224
6 0.260632995
7 0.062998008
8 0.135923613
9 -7.213887201
10 0.26610021
11 -0.081985411
12 0.097038369
13 -0.160927687
14 0.025626033
15 0.242435117
16 -0.198993575
17 0.267910151
18 0.003851139
19 -0.362262009
20 -0.014511449
21 -0.011519575
24 -0.137525114
25 0.688698333

Total number of rows: 58753

Table truncated, full table size 1044 Kbytes.

Supplementary file Size Download File type/resource
GSM1332748_81_2_3.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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