NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1332742 Query DataSets for GSM1332742
Status Public on Feb 22, 2014
Title terminal_ileum_patient22
Sample type RNA
 
Source name Terminal ileum
Organism Homo sapiens
Characteristics tissue: Intestinal mucosa
gender: Male
age: 51
Treatment protocol N/A
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol Pinch biopsy that was snap frozen in Trizol for RNA extraction using chloroform purification and isopropanol extraction and purification using QIAgen Rneasy Mini Kit
Label Cy3
Label protocol Labeling was performed with Cy3 according to the manufacturer protoocl for one color microarray based gene expression analysis (Agilent)
 
Hybridization protocol Cy3 labeled, linearly amplified complementary RNA was synthesized and hybridized to Sureprint G3 Human Gene Expression 8 x 60K (Agilent) microarrays according to manufacturer's protocol.
Scan protocol Arrays were scanned using a High Resolution C scanner
Data processing Raw signal intensities were extracted with feature extraction v10.7 software with the protocol GE1_107_Sep09 and Grid 028004_D_F_20110819, to obtain spatially detrended signal intensities.
 
Submission date Feb 21, 2014
Last update date Feb 22, 2014
Contact name Mei San Tang
Organization name Washington University in St Louis
Department Pathology & Immunology
Street address 660 S Euclid, Campus Box 8118
City Saint Louis
State/province MO
ZIP/Postal code 63108
Country USA
 
Platform ID GPL13607
Series (1)
GSE55258 Th17, Th22 and Treg cells are enriched in the healthy human cecum

Data table header descriptions
ID_REF
VALUE Expression intensities were log2-transformed, quantile normalized and median-centered.

Data table
ID_REF VALUE
4 0.03018154
5 -0.221896313
6 0.004114351
7 0.526752314
8 -0.068266381
9 -7.420246847
10 -0.01721541
11 0.060223152
12 0.387788791
13 0.016774883
14 -0.018607729
15 -1.289874455
16 -0.256676942
17 0.037070609
18 -0.26852718
19 -0.550138278
20 -0.105883871
21 0.555444753
24 0.20036702
25 -1.178524827

Total number of rows: 58753

Table truncated, full table size 1046 Kbytes.




Supplementary file Size Download File type/resource
GSM1332742_81_1_1.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap