|
| Status |
Public on Feb 22, 2014 |
| Title |
sigmoid_colon_patient10 |
| Sample type |
RNA |
| |
|
| Source name |
Sigmoid colon
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Intestinal mucosa
gender: Female
age: 57
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Pinch biopsy that was snap frozen in Trizol for RNA extraction using chloroform purification and isopropanol extraction and purification using QIAgen Rneasy Mini Kit
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed with Cy3 according to the manufacturer protoocl for one color microarray based gene expression analysis (Agilent)
|
| |
|
| Hybridization protocol |
Cy3 labeled, linearly amplified complementary RNA was synthesized and hybridized to Sureprint G3 Human Gene Expression 8 x 60K (Agilent) microarrays according to manufacturer's protocol.
|
| Scan protocol |
Arrays were scanned using a High Resolution C scanner
|
| Data processing |
Raw signal intensities were extracted with feature extraction v10.7 software with the protocol GE1_107_Sep09 and Grid 028004_D_F_20110819, to obtain spatially detrended signal intensities.
|
| |
|
| Submission date |
Feb 21, 2014 |
| Last update date |
Feb 22, 2014 |
| Contact name |
Mei San Tang |
| Organization name |
Washington University in St Louis
|
| Department |
Pathology & Immunology
|
| Street address |
660 S Euclid, Campus Box 8118
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63108 |
| Country |
USA |
| |
|
| Platform ID |
GPL13607 |
| Series (1) |
| GSE55258 |
Th17, Th22 and Treg cells are enriched in the healthy human cecum |
|
