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Sample GSM1332736 Query DataSets for GSM1332736
Status Public on Feb 22, 2014
Title sigmoid_colon_patient10
Sample type RNA
Source name Sigmoid colon
Organism Homo sapiens
Characteristics tissue: Intestinal mucosa
gender: Female
age: 57
Treatment protocol N/A
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol Pinch biopsy that was snap frozen in Trizol for RNA extraction using chloroform purification and isopropanol extraction and purification using QIAgen Rneasy Mini Kit
Label Cy3
Label protocol Labeling was performed with Cy3 according to the manufacturer protoocl for one color microarray based gene expression analysis (Agilent)
Hybridization protocol Cy3 labeled, linearly amplified complementary RNA was synthesized and hybridized to Sureprint G3 Human Gene Expression 8 x 60K (Agilent) microarrays according to manufacturer's protocol.
Scan protocol Arrays were scanned using a High Resolution C scanner
Data processing Raw signal intensities were extracted with feature extraction v10.7 software with the protocol GE1_107_Sep09 and Grid 028004_D_F_20110819, to obtain spatially detrended signal intensities.
Submission date Feb 21, 2014
Last update date Feb 22, 2014
Contact name Mei San Tang
Organization name Washington University in St Louis
Department Pathology & Immunology
Street address 660 S Euclid, Campus Box 8118
City Saint Louis
State/province MO
ZIP/Postal code 63108
Country USA
Platform ID GPL13607
Series (1)
GSE55258 Th17, Th22 and Treg cells are enriched in the healthy human cecum

Data table header descriptions
VALUE Expression intensities were log2-transformed, quantile normalized and median-centered.

Data table
4 -0.028599421
5 0.051420552
6 -0.337909919
7 -0.072392795
8 -0.734197908
9 0.195790568
10 -0.271854126
11 0.030220152
12 -0.328300944
13 -0.265334938
14 -0.605669662
15 1.063064325
16 -0.324453513
17 -0.128506262
18 -0.130384873
19 -0.08101705
20 0.238876295
21 -0.523545742
24 0.049099395
25 -0.108688235

Total number of rows: 58753

Table truncated, full table size 1044 Kbytes.

Supplementary file Size Download File type/resource
GSM1332736_80_1_3.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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