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Sample GSM1332732 Query DataSets for GSM1332732
Status Public on Feb 22, 2014
Title sigmoid_colon_patient9
Sample type RNA
Source name Sigmoid colon
Organism Homo sapiens
Characteristics tissue: Intestinal mucosa
gender: Male
age: 51
Treatment protocol N/A
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol Pinch biopsy that was snap frozen in Trizol for RNA extraction using chloroform purification and isopropanol extraction and purification using QIAgen Rneasy Mini Kit
Label Cy3
Label protocol Labeling was performed with Cy3 according to the manufacturer protoocl for one color microarray based gene expression analysis (Agilent)
Hybridization protocol Cy3 labeled, linearly amplified complementary RNA was synthesized and hybridized to Sureprint G3 Human Gene Expression 8 x 60K (Agilent) microarrays according to manufacturer's protocol.
Scan protocol Arrays were scanned using a High Resolution C scanner
Data processing Raw signal intensities were extracted with feature extraction v10.7 software with the protocol GE1_107_Sep09 and Grid 028004_D_F_20110819, to obtain spatially detrended signal intensities.
Submission date Feb 21, 2014
Last update date Feb 22, 2014
Contact name Mei San Tang
Organization name Washington University in St Louis
Department Pathology & Immunology
Street address 660 S Euclid, Campus Box 8118
City Saint Louis
State/province MO
ZIP/Postal code 63108
Country USA
Platform ID GPL13607
Series (1)
GSE55258 Th17, Th22 and Treg cells are enriched in the healthy human cecum

Data table header descriptions
VALUE Expression intensities were log2-transformed, quantile normalized and median-centered.

Data table
4 -0.011888124
5 -0.404346224
6 -0.165551397
7 0.016359515
8 0.705306294
9 -0.276622457
10 -0.082311038
11 0.254132112
12 -0.246137882
13 0.09963346
14 0.593051669
15 -1.885061218
16 0.401967866
17 -0.475791644
18 0.012028081
19 0.799106917
20 0.297362699
21 0.020827209
24 0.252922205
25 0.92799597

Total number of rows: 58753

Table truncated, full table size 1046 Kbytes.

Supplementary file Size Download File type/resource
GSM1332732_79_2_3.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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