GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1332227 Query DataSets for GSM1332227
Status Public on Feb 21, 2014
Title Synovial tissue from rheumatoid arthritis joint 7 (RA_7)
Sample type RNA
Source name synovial tissue from rheumatoid arthritis joint
Organism Homo sapiens
Characteristics disease state: rheumatoid arthritis
tissue: synovial tissue isolated from joint
Treatment protocol tissues were collected from joint replacement surgery in OA patients and synovecomy surgery in RA patients, normal synovium was collected early post mortem from macroscopically normal knee joints
Growth protocol tissues were immediately frozen in liquid nitrogen for subsequent RNA-extraction
Extracted molecule total RNA
Extraction protocol RNA was collected by using the RNeasy mini kit (Qiagen)
Label biotin
Label protocol 3-5 µg of total RNA was amplified and labelled using the GeneChip® one-cycle target labelling and control reagents (Affymetrix)
Hybridization protocol 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Description Gene expression data of synovial tissue isolated from rheumatoid arthritis joint number 7, labelled as RA_7
Data processing GCOS
Submission date Feb 21, 2014
Last update date Feb 21, 2014
Contact name Thomas Häupl
Phone +49 30 450513293
Organization name Charité
Department Rheumatology
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
Platform ID GPL96
Series (1)
GSE55235 Identification of rheumatoid arthritis and osteoarthritis patients by transcriptome-based rule set generation

Data table header descriptions
VALUE MAS 5.0 signal

Data table
AFFX-BioB-5_at 98.7 P 0.036569
AFFX-BioB-M_at 100.7 P 0.013811
AFFX-BioB-3_at 19.5 A 0.195266
AFFX-BioC-5_at 231.4 P 0.001248
AFFX-BioC-3_at 177.2 P 0.000581
AFFX-BioDn-5_at 166.5 P 0.000558
AFFX-BioDn-3_at 1904 P 0.000754
AFFX-CreX-5_at 2530.5 P 0.000044
AFFX-CreX-3_at 3648.2 P 0.000044
AFFX-DapX-5_at 1.9 A 0.617401
AFFX-DapX-M_at 2.1 A 0.699565
AFFX-DapX-3_at 2.3 A 0.878399
AFFX-LysX-5_at 1.8 A 0.860518
AFFX-LysX-M_at 4.9 A 0.724854
AFFX-LysX-3_at 1.8 A 0.724854
AFFX-PheX-5_at 2.1 A 0.945787
AFFX-PheX-M_at 1.6 A 0.897835
AFFX-PheX-3_at 7.6 A 0.760937
AFFX-ThrX-5_at 2.8 A 0.937071
AFFX-ThrX-M_at 1.9 A 0.941556

Total number of rows: 22283

Table truncated, full table size 582 Kbytes.

Supplementary file Size Download File type/resource
GSM1332227_RA_7_Syn_133A.cel.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap