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Sample GSM1332210 Query DataSets for GSM1332210
Status Public on Feb 21, 2014
Title Synovial tisssue from healthy joint 10 (ND_10)
Sample type RNA
Source name synovial tissue from healthy joint
Organism Homo sapiens
Characteristics disease state: healthy control
tissue: synovial tissue isolated from joint
Treatment protocol tissues were collected from joint replacement surgery in OA patients and synovecomy surgery in RA patients, normal synovium was collected early post mortem from macroscopically normal knee joints
Growth protocol tissues were immediately frozen in liquid nitrogen for subsequent RNA-extraction
Extracted molecule total RNA
Extraction protocol RNA was collected by using the RNeasy mini kit (Qiagen)
Label biotin
Label protocol 3-5 µg of total RNA was amplified and labelled using the GeneChip® one-cycle target labelling and control reagents (Affymetrix)
Hybridization protocol 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Description Gene expression data of synovial tissue isolated from healthy joint number 10, labelled as ND_10
Data processing GCOS
Submission date Feb 21, 2014
Last update date Feb 21, 2014
Contact name Thomas Häupl
Phone +49 30 450513293
Organization name Charité
Department Rheumatology
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
Platform ID GPL96
Series (1)
GSE55235 Identification of rheumatoid arthritis and osteoarthritis patients by transcriptome-based rule set generation

Data table header descriptions
VALUE MAS 5.0 signal

Data table
AFFX-BioB-5_at 113.2 P 0.039661
AFFX-BioB-M_at 132.5 P 0.003595
AFFX-BioB-3_at 74.1 A 0.083592
AFFX-BioC-5_at 274.3 P 0.000857
AFFX-BioC-3_at 141 P 0.000754
AFFX-BioDn-5_at 222.5 P 0.001796
AFFX-BioDn-3_at 1554.6 P 0.000857
AFFX-CreX-5_at 2177.7 P 0.000044
AFFX-CreX-3_at 2984.9 P 0.000044
AFFX-DapX-5_at 9.8 A 0.195266
AFFX-DapX-M_at 24.6 A 0.147939
AFFX-DapX-3_at 3.5 A 0.904333
AFFX-LysX-5_at 2.7 A 0.868639
AFFX-LysX-M_at 11.6 A 0.737173
AFFX-LysX-3_at 9.7 A 0.287743
AFFX-PheX-5_at 2 A 0.891021
AFFX-PheX-M_at 4.5 A 0.852061
AFFX-PheX-3_at 9.6 A 0.804734
AFFX-ThrX-5_at 4.4 A 0.883887
AFFX-ThrX-M_at 4.2 A 0.686277

Total number of rows: 22283

Table truncated, full table size 584 Kbytes.

Supplementary file Size Download File type/resource
GSM1332210_ND_10_Syn_133A.cel.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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