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Sample GSM1332207 Query DataSets for GSM1332207
Status Public on Feb 21, 2014
Title Synovial tisssue from healthy joint 7 (ND_7)
Sample type RNA
Source name synovial tissue from healthy joint
Organism Homo sapiens
Characteristics disease state: healthy control
tissue: synovial tissue isolated from joint
Treatment protocol tissues were collected from joint replacement surgery in OA patients and synovecomy surgery in RA patients, normal synovium was collected early post mortem from macroscopically normal knee joints
Growth protocol tissues were immediately frozen in liquid nitrogen for subsequent RNA-extraction
Extracted molecule total RNA
Extraction protocol RNA was collected by using the RNeasy mini kit (Qiagen)
Label biotin
Label protocol 3-5 µg of total RNA was amplified and labelled using the GeneChip® one-cycle target labelling and control reagents (Affymetrix)
Hybridization protocol 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Description Gene expression data of synovial tissue isolated from healthy joint number 7, labelled as ND_7
Data processing GCOS
Submission date Feb 21, 2014
Last update date Feb 21, 2014
Contact name Thomas Häupl
Phone +49 30 450513293
Organization name Charité
Department Rheumatology
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
Platform ID GPL96
Series (1)
GSE55235 Identification of rheumatoid arthritis and osteoarthritis patients by transcriptome-based rule set generation

Data table header descriptions
VALUE MAS 5.0 signal

Data table
AFFX-BioB-5_at 125.4 P 0.010317
AFFX-BioB-M_at 195.5 P 0.013811
AFFX-BioB-3_at 89 P 0.020022
AFFX-BioC-5_at 275.2 P 0.000857
AFFX-BioC-3_at 235.2 P 0.000297
AFFX-BioDn-5_at 256.7 P 0.001451
AFFX-BioDn-3_at 2140.5 P 0.000754
AFFX-CreX-5_at 3432 P 0.000044
AFFX-CreX-3_at 4421.7 P 0.000044
AFFX-DapX-5_at 1.2 A 0.824672
AFFX-DapX-M_at 5.4 A 0.532074
AFFX-DapX-3_at 1.4 A 0.960339
AFFX-LysX-5_at 13 A 0.5
AFFX-LysX-M_at 5.2 A 0.749204
AFFX-LysX-3_at 2 A 0.544587
AFFX-PheX-5_at 1.3 A 0.916408
AFFX-PheX-M_at 1.9 A 0.916408
AFFX-PheX-3_at 8.6 A 0.659339
AFFX-ThrX-5_at 3.5 A 0.868639
AFFX-ThrX-M_at 3.9 A 0.699394

Total number of rows: 22283

Table truncated, full table size 582 Kbytes.

Supplementary file Size Download File type/resource
GSM1332207_ND_7_Syn_133A.cel.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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