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Sample GSM1332206 Query DataSets for GSM1332206
Status Public on Feb 21, 2014
Title Synovial tisssue from healthy joint 6 (ND_6)
Sample type RNA
Source name synovial tissue from healthy joint
Organism Homo sapiens
Characteristics disease state: healthy control
tissue: synovial tissue isolated from joint
Treatment protocol tissues were collected from joint replacement surgery in OA patients and synovecomy surgery in RA patients, normal synovium was collected early post mortem from macroscopically normal knee joints
Growth protocol tissues were immediately frozen in liquid nitrogen for subsequent RNA-extraction
Extracted molecule total RNA
Extraction protocol RNA was collected by using the RNeasy mini kit (Qiagen)
Label biotin
Label protocol 3-5 µg of total RNA was amplified and labelled using the GeneChip® one-cycle target labelling and control reagents (Affymetrix)
Hybridization protocol 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Description Gene expression data of synovial tissue isolated from healthy joint number 6, labelled as ND_6
Data processing GCOS
Submission date Feb 21, 2014
Last update date Feb 21, 2014
Contact name Thomas Häupl
Phone +49 30 450513293
Organization name Charité
Department Rheumatology
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
Platform ID GPL96
Series (1)
GSE55235 Identification of rheumatoid arthritis and osteoarthritis patients by transcriptome-based rule set generation

Data table header descriptions
VALUE MAS 5.0 signal

Data table
AFFX-BioB-5_at 139.9 P 0.003595
AFFX-BioB-M_at 191 P 0.001113
AFFX-BioB-3_at 110.3 P 0.012547
AFFX-BioC-5_at 356 P 0.000297
AFFX-BioC-3_at 326.1 P 0.00011
AFFX-BioDn-5_at 314.5 P 0.000078
AFFX-BioDn-3_at 1798.2 P 0.000147
AFFX-CreX-5_at 4244.7 P 0.000044
AFFX-CreX-3_at 5426.4 P 0.000044
AFFX-DapX-5_at 1.3 A 0.699394
AFFX-DapX-M_at 4.1 A 0.544587
AFFX-DapX-3_at 1.3 A 0.941556
AFFX-LysX-5_at 1.4 A 0.574038
AFFX-LysX-M_at 2.3 A 0.960339
AFFX-LysX-3_at 1.1 A 0.659339
AFFX-PheX-5_at 1.9 A 0.932322
AFFX-PheX-M_at 1.4 A 0.897835
AFFX-PheX-3_at 4.7 A 0.883887
AFFX-ThrX-5_at 2.9 A 0.834139
AFFX-ThrX-M_at 1.2 A 0.883887

Total number of rows: 22283

Table truncated, full table size 581 Kbytes.

Supplementary file Size Download File type/resource
GSM1332206_ND_6_Syn_133A.cel.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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