GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1332205 Query DataSets for GSM1332205
Status Public on Feb 21, 2014
Title Synovial tisssue from healthy joint 5 (ND_5)
Sample type RNA
Source name synovial tissue from healthy joint
Organism Homo sapiens
Characteristics disease state: healthy control
tissue: synovial tissue isolated from joint
Treatment protocol tissues were collected from joint replacement surgery in OA patients and synovecomy surgery in RA patients, normal synovium was collected early post mortem from macroscopically normal knee joints
Growth protocol tissues were immediately frozen in liquid nitrogen for subsequent RNA-extraction
Extracted molecule total RNA
Extraction protocol RNA was collected by using the RNeasy mini kit (Qiagen)
Label biotin
Label protocol 3-5 µg of total RNA was amplified and labelled using the GeneChip® one-cycle target labelling and control reagents (Affymetrix)
Hybridization protocol 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Description Gene expression data of synovial tissue isolated from healthy joint number 5, labelled as ND_5
Data processing GCOS
Submission date Feb 21, 2014
Last update date Feb 21, 2014
Contact name Thomas Häupl
Phone +49 30 450513293
Organization name Charité
Department Rheumatology
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
Platform ID GPL96
Series (1)
GSE55235 Identification of rheumatoid arthritis and osteoarthritis patients by transcriptome-based rule set generation

Data table header descriptions
VALUE MAS 5.0 signal

Data table
AFFX-BioB-5_at 132.1 P 0.00762
AFFX-BioB-M_at 115.2 P 0.002023
AFFX-BioB-3_at 58.2 P 0.042962
AFFX-BioC-5_at 252.7 P 0.000754
AFFX-BioC-3_at 184.6 P 0.000195
AFFX-BioDn-5_at 230.3 P 0.000419
AFFX-BioDn-3_at 1639.7 P 0.000662
AFFX-CreX-5_at 2757.6 P 0.000044
AFFX-CreX-3_at 3599 P 0.000044
AFFX-DapX-5_at 1.3 A 0.772364
AFFX-DapX-M_at 2.4 A 0.452897
AFFX-DapX-3_at 0.9 A 0.986189
AFFX-LysX-5_at 4.2 A 0.772364
AFFX-LysX-M_at 4.3 A 0.772364
AFFX-LysX-3_at 1.4 A 0.686277
AFFX-PheX-5_at 1.2 A 0.969024
AFFX-PheX-M_at 1 A 0.883887
AFFX-PheX-3_at 6.9 A 0.749204
AFFX-ThrX-5_at 4.4 A 0.876428
AFFX-ThrX-M_at 2.6 A 0.852061

Total number of rows: 22283

Table truncated, full table size 582 Kbytes.

Supplementary file Size Download File type/resource
GSM1332205_ND_5_Syn_133A.cel.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap