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Sample GSM1331674 Query DataSets for GSM1331674
Status Public on May 24, 2014
Title leaf at T0, biological rep1
Sample type RNA
Source name Poplar leaves under normal CO2 concentration
Organism (Populus tomentosa x Populus bolleana) x Populus tomentosa
Characteristics treatment: normal CO2 concentration
tissue: leaf
Treatment protocol The three different CO2 concentration treatments were: T0 treatment (385 ppm, day 25˚C/night 20˚C), T1 treatment (550 ppm, day 28˚C/night 23˚C) and T2 treatment (720 ppm, day 31˚C/night 26˚C).
Growth protocol Homogeneous 20 cm long, woody-stem cuttings of triploid white poplar were planted in 20 cm*26 cm*34 cm plastic pots. After growth for about three months, twenty randomly selected pots were moved into three controlled environment chambers (Beijing, China).Ten days later, they were subjected to different CO2 concentrations treatments three months.
Extracted molecule total RNA
Extraction protocol total RNA
Label biotin
Label protocol biotin
Hybridization protocol Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
Scan protocol Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
Description Gene expression data from leaves under normal CO2 concentration
Data processing Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
Submission date Feb 20, 2014
Last update date May 24, 2014
Contact name Liu Juanjuan
Organization name Chinese Academy of Forestry
Department Research Institute of Forestry
Street address XiangShan Road, HaiDian District
City Bjing
ZIP/Postal code 100091
Country China
Platform ID GPL4359
Series (1)
GSE55216 Expression data from leaf of (P. tomentosa x P. bolleana) x P. tomentosa under elevated CO2 concentrations

Data table header descriptions
VALUE MAS5.0 signal intensity

Data table
AFFX-BioB-3_at 789.3109 P 4.43E-05
AFFX-BioB-5_at 716.5779 P 5.17E-05
AFFX-BioB-M_at 1199.959 P 4.43E-05
AFFX-BioC-3_at 1935.392 P 4.43E-05
AFFX-BioC-5_at 2036.567 P 4.43E-05
AFFX-BioDn-3_at 8578.362 P 4.43E-05
AFFX-BioDn-5_at 5161.693 P 4.43E-05
AFFX-CreX-3_at 25766.84 P 4.43E-05
AFFX-CreX-5_at 23391.65 P 5.17E-05
AFFX-DapX-3_at 2727.208 P 4.43E-05
AFFX-DapX-5_at 682.3363 P 4.43E-05
AFFX-DapX-M_at 2218.667 P 5.17E-05
AFFX-LysX-3_at 337.0455 P 5.17E-05
AFFX-LysX-5_at 96.23492 P 9.45E-05
AFFX-LysX-M_at 160.0742 P 0.000445901
AFFX-PheX-3_at 345.3663 P 4.43E-05
AFFX-PheX-5_at 168.0132 P 6.02E-05
AFFX-PheX-M_at 249.8159 P 7.01E-05
AFFX-Ptp-actin-3_s_at 11499.33 P 0.000244141
AFFX-Ptp-actin-5_at 6572.877 P 0.000244141

Total number of rows: 61413

Table truncated, full table size 2598 Kbytes.

Supplementary file Size Download File type/resource
GSM1331674_BH2010392-10_131L9_Poplar_.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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