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Sample GSM132956 Query DataSets for GSM132956
Status Public on Sep 15, 2006
Title SKI-DLCL cells_Aplidin 1.5 nM AraC 5nM_24h_rpt2
Sample type RNA
 
Source name SKI-DLCL cells
Organism Homo sapiens
Characteristics SKI-DLCL cells were treated with Aplidin 1.5nM and AraC 5.0 nM combination for 24h. Total RNA will be extracted.
Biomaterial provider own
Treatment protocol Combination of Aplidin 1.5 nM and AraC 5 nM
Growth protocol Cells were grown under standard cell culture condition (95% air, 5% CO2, 37C)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNeasy kit from Qiagen.
Label Biotin
Label protocol Synthesis of Biotin-Labeled cRNA/Purification

ENZO BioArray RNA Transcript Labeling Kit
GeneChip Sample Cleanup Module

Miscellaneous Reagents/Supplies

100% ethanol ? room temperature
80% ethanol ? room temperature
Water, Molecular Biology Grade or DEPC-treated
Ethidium Bromide
Sterile, RNase-free, microcentrifuge tubes, 1.5mL
Micropipettors
Sterile-barrier, RNase-free pipette tips
Mini agarose gel electrophoresis unit with appropriate buffers
UV Spectrophotometer
Cooling water bath
 
Hybridization protocol Hybridization

Water, Molecular Biology Grade or DEPC-treated
Acetylated Bovine Serum Albumin (BSA) solution (50 mg/mL)
Herring Sperm DNA
GeneChip Eukaryotic Hybridization Control Kit
5M NaCl, RNase-free, DNase-free
MES Free Acid Monohydrate
MES Sodium Salt
EDTA Disodium Salt

Miscellaneous Reagents/Supplies

Surfact-Amps 20 (Tween-20), 10%
Hybridization Oven 640
Sterile, RNase-free, microcentrifuge tubes, 1.5mL
Micropipettors
Sterile-barrier pipette tips and non-barrier tips
Heatblock
Scan protocol Washing, Staining, and Scanning

Water, Molecular Biology Grade
Distilled water
Acetylated Bovine Serum Albumin (BSA) solution (50 mg/mL)
R-Phycoerythrin Streptavidin
20X SSPE (3M NaCl, 0.2M NaH2PO4, 0.02M EDTA)
Goat IgG, Reagent Grade
Anti-streptavidin antibody (goat), biotinylated
10% surfact-Amps 20 (Tween-20)
Description Samples were processed in CINJ Core Expression Array Facility according to Affymetrix protocol.
Data processing scanned fluorescence intensity, no normalization
 
Submission date Aug 31, 2006
Last update date Dec 23, 2010
Contact name Pravin Mishra
E-mail(s) mishrapr@umdnj.edu
Organization name UMDNJ
Department Pharmacology
Lab Bertino/Banerjee
Street address 195 Little Albany St.
City New Brunswick
State/province NJ
ZIP/Postal code 08904
Country USA
 
Platform ID GPL571
Series (1)
GSE5681 Aplidin synergizes with cytosine arabinoside: functional relevance of mitochondria in Aplidin-induced cytotoxicity

Data table header descriptions
ID_REF
VALUE fluorescence intensity values

Data table
ID_REF VALUE
AFFX-BioB-5_at 89.7
AFFX-BioB-M_at 140.7
AFFX-BioB-3_at 81.8
AFFX-BioC-5_at 296.2
AFFX-BioC-3_at 388.6
AFFX-BioDn-5_at 696.8
AFFX-BioDn-3_at 1375.5
AFFX-CreX-5_at 3428.2
AFFX-CreX-3_at 3912.3
AFFX-DapX-5_at 2.7
AFFX-DapX-M_at 2.8
AFFX-DapX-3_at 5
AFFX-LysX-5_at 1.5
AFFX-LysX-M_at 0.7
AFFX-LysX-3_at 3.4
AFFX-PheX-5_at 0.5
AFFX-PheX-M_at 0.4
AFFX-PheX-3_at 4.1
AFFX-ThrX-5_at 0.8
AFFX-ThrX-M_at 0.7

Total number of rows: 22277

Table truncated, full table size 342 Kbytes.




Supplementary file Size Download File type/resource
GSM132956.CEL.gz 3.2 Mb (ftp)(http) CEL

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