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Sample GSM1328236 Query DataSets for GSM1328236
Status Public on Jun 05, 2015
Title Patient 15 - skin-week 7 visit
Sample type RNA
 
Source name human skin
Organism Homo sapiens
Characteristics treatment: fresolimumab one dose 5 mg/kg
Extracted molecule total RNA
Extraction protocol Skin biopseie placed in RNAlater at bedside were stored at -80 degrees C until processing. RNA was exrtacted by homogenization in RNAzol and prifying over Rneasy columns
Label biotin
Label protocol Biotin labeling was performed using the 3' IVT Express Kit (Affymetrix, Santa Clara, CA) according to the manufacturer's protocol.
 
Hybridization protocol The labeled, fragmented DNA was hybridized to the GeneChip HG-U133A 2.0 Array (Affymetrix, Santa Clara, CA) for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450
Scan protocol After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus (Affymetrix, Santa Clara, CA).
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date Feb 14, 2014
Last update date Jun 05, 2015
Contact name Robert Lafyatis
E-mail(s) rlafyatis@gmail.com
Phone 6176385388
Organization name Boston University School of Medicine
Department Rheumatology
Street address 72 E Concord St
City Boston
State/province MA
ZIP/Postal code 02118
Country USA
 
Platform ID GPL571
Series (1)
GSE55036 Expression data from skin biopsies in pateints with systemic sclerosis treated with anti-TGF-beta, fresolimumab

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 1042.611
1053_at 330.0776
117_at 146.482
121_at 275.9306
1255_g_at 13.22091
1294_at 151.4261
1316_at 41.99305
1320_at 129.2787
1405_i_at 683.5952
1431_at 140.2078
1438_at 78.26186
1487_at 276.3472
1494_f_at 33.4213
1598_g_at 1210.228
160020_at 455.3641
1729_at 321.9259
1773_at 63.25517
177_at 175.0232
179_at 133.4411
1861_at 184.5364

Total number of rows: 22277

Table truncated, full table size 431 Kbytes.




Supplementary file Size Download File type/resource
GSM1328236_GC15_SD12-86.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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