NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1328228 Query DataSets for GSM1328228
Status Public on Jun 05, 2015
Title Patient 12 - skin-week 3/4 visit
Sample type RNA
 
Source name human skin
Organism Homo sapiens
Characteristics treatment: fresolimumab one dose 5 mg/kg
Extracted molecule total RNA
Extraction protocol Skin biopseie placed in RNAlater at bedside were stored at -80 degrees C until processing. RNA was exrtacted by homogenization in RNAzol and prifying over Rneasy columns
Label biotin
Label protocol Biotin labeling was performed using the 3' IVT Express Kit (Affymetrix, Santa Clara, CA) according to the manufacturer's protocol.
 
Hybridization protocol The labeled, fragmented DNA was hybridized to the GeneChip HG-U133A 2.0 Array (Affymetrix, Santa Clara, CA) for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450
Scan protocol After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus (Affymetrix, Santa Clara, CA).
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date Feb 14, 2014
Last update date Jun 05, 2015
Contact name Robert Lafyatis
E-mail(s) rlafyatis@gmail.com
Phone 6176385388
Organization name Boston University School of Medicine
Department Rheumatology
Street address 72 E Concord St
City Boston
State/province MA
ZIP/Postal code 02118
Country USA
 
Platform ID GPL571
Series (1)
GSE55036 Expression data from skin biopsies in pateints with systemic sclerosis treated with anti-TGF-beta, fresolimumab

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 1150.787
1053_at 245.7178
117_at 96.00868
121_at 255.377
1255_g_at 12.09451
1294_at 145.3703
1316_at 90.43464
1320_at 154.1702
1405_i_at 501.7715
1431_at 95.35154
1438_at 124.6912
1487_at 298.921
1494_f_at 42.78818
1598_g_at 1761.077
160020_at 518.6097
1729_at 341.7194
1773_at 54.27188
177_at 153.453
179_at 70.23144
1861_at 156.7085

Total number of rows: 22277

Table truncated, full table size 431 Kbytes.




Supplementary file Size Download File type/resource
GSM1328228_GC12_SD12-69.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap