NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1328210 Query DataSets for GSM1328210
Status Public on Jun 05, 2015
Title Patient 5 - skin-baseline visit
Sample type RNA
 
Source name human skin
Organism Homo sapiens
Characteristics treatment: fresolimumab two doses 1 mg/kg at baseline and 3 wks
Extracted molecule total RNA
Extraction protocol Skin biopseie placed in RNAlater at bedside were stored at -80 degrees C until processing. RNA was exrtacted by homogenization in RNAzol and prifying over Rneasy columns
Label biotin
Label protocol Biotin labeling was performed using the 3' IVT Express Kit (Affymetrix, Santa Clara, CA) according to the manufacturer's protocol.
 
Hybridization protocol The labeled, fragmented DNA was hybridized to the GeneChip HG-U133A 2.0 Array (Affymetrix, Santa Clara, CA) for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450
Scan protocol After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus (Affymetrix, Santa Clara, CA).
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date Feb 14, 2014
Last update date Jun 05, 2015
Contact name Robert Lafyatis
E-mail(s) rlafyatis@gmail.com
Phone 6176385388
Organization name Boston University School of Medicine
Department Rheumatology
Street address 72 E Concord St
City Boston
State/province MA
ZIP/Postal code 02118
Country USA
 
Platform ID GPL571
Series (1)
GSE55036 Expression data from skin biopsies in pateints with systemic sclerosis treated with anti-TGF-beta, fresolimumab

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 1096.928
1053_at 246.5478
117_at 159.6033
121_at 180.9459
1255_g_at 21.46837
1294_at 143.4761
1316_at 110.184
1320_at 103.8914
1405_i_at 447.57
1431_at 84.10258
1438_at 103.5404
1487_at 299.708
1494_f_at 44.12922
1598_g_at 2259.065
160020_at 512.3315
1729_at 416.0564
1773_at 55.00932
177_at 172.9357
179_at 79.50033
1861_at 179.0518

Total number of rows: 22277

Table truncated, full table size 431 Kbytes.




Supplementary file Size Download File type/resource
GSM1328210_SD-11.17.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap