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Sample GSM1328205 Query DataSets for GSM1328205
Status Public on Jun 05, 2015
Title Patient 3 - skin-baseline visit
Sample type RNA
 
Source name human skin
Organism Homo sapiens
Characteristics treatment: fresolimumab two doses 1 mg/kg at baseline and 3 wks
Extracted molecule total RNA
Extraction protocol Skin biopseie placed in RNAlater at bedside were stored at -80 degrees C until processing. RNA was exrtacted by homogenization in RNAzol and prifying over Rneasy columns
Label biotin
Label protocol Biotin labeling was performed using the 3' IVT Express Kit (Affymetrix, Santa Clara, CA) according to the manufacturer's protocol.
 
Hybridization protocol The labeled, fragmented DNA was hybridized to the GeneChip HG-U133A 2.0 Array (Affymetrix, Santa Clara, CA) for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450
Scan protocol After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus (Affymetrix, Santa Clara, CA).
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date Feb 14, 2014
Last update date Jun 05, 2015
Contact name Robert Lafyatis
E-mail(s) rlafyatis@gmail.com
Phone 6176385388
Organization name Boston University School of Medicine
Department Rheumatology
Street address 72 E Concord St
City Boston
State/province MA
ZIP/Postal code 02118
Country USA
 
Platform ID GPL571
Series (1)
GSE55036 Expression data from skin biopsies in pateints with systemic sclerosis treated with anti-TGF-beta, fresolimumab

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 1130.888
1053_at 341.5604
117_at 152.5279
121_at 117.5609
1255_g_at 21.35621
1294_at 84.44072
1316_at 71.47104
1320_at 75.67353
1405_i_at 120.3708
1431_at 212.1361
1438_at 87.41795
1487_at 407.0688
1494_f_at 17.10986
1598_g_at 1822.269
160020_at 566.3898
1729_at 357.7751
1773_at 44.09595
177_at 247.7499
179_at 70.1559
1861_at 185.6352

Total number of rows: 22277

Table truncated, full table size 431 Kbytes.




Supplementary file Size Download File type/resource
GSM1328205_SD-11-13.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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