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Sample GSM1325182 Query DataSets for GSM1325182
Status Public on Aug 04, 2014
Title NE_iPSC2
Sample type RNA
 
Source name Neuroectodermal cells differentiated from human induced pluripotent stem cells 2
Organism Homo sapiens
Characteristics cell type: Neuroectodermal cells differentiated from human induced pluripotent stem cells 2
Extracted molecule total RNA
Extraction protocol Total RNA was isolated by Trizol reagent (Life Technologies) according to the manufacturer's instruction.
Label Cy3
Label protocol Agilent standard protocol
 
Hybridization protocol Agilent standard protocol
Scan protocol Agilent standard protocol
Description Neuroectodermal cells differentiated from human induced pluripotent stem cells 2
Data processing The data were analyzed using the Gene Spring GX 11.5.1 software program (Agilent Technologies).The data processing was performed as follows; (i) Threshold raw signals were set to 1.0, (ii) Log base 2 transformation was performed, (iii) 75th percentile normalization was chosen as the normalized algorithm.
 
Submission date Feb 10, 2014
Last update date Aug 04, 2014
Contact name Kazutoshi Takahashi
E-mail(s) kazu@cira.kyoto-u.ac.jp
Organization name Kyoto University
Department Center for iPS Cell Research and Application
Street address 53 Kawahara-cho, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
 
Platform ID GPL14550
Series (1)
GSE54848 Critical role of transient activation of human endogenous retroviruses during reprogramming toward pluripotency

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
(+)E1A_r60_1 9.274462
(+)E1A_r60_3 -7.551725
(+)E1A_r60_a104 -7.5373564
(+)E1A_r60_a107 -3.4758291
(+)E1A_r60_a135 -0.36938763
(+)E1A_r60_a20 0.74862003
(+)E1A_r60_a22 2.3874063
(+)E1A_r60_a97 4.609626
(+)E1A_r60_n11 6.717558
(+)E1A_r60_n9 7.5429096
3xSLv1 -7.7823663
A_19_P00315452 -7.8482633
A_19_P00315459 -2.1618032
A_19_P00315469 -7.877048
A_19_P00315473 -7.6369753
A_19_P00315482 -4.6701145
A_19_P00315490 -7.7932587
A_19_P00315492 -5.412595
A_19_P00315493 -0.5685158
A_19_P00315496 -7.997179

Total number of rows: 42545

Table truncated, full table size 1001 Kbytes.




Supplementary file Size Download File type/resource
GSM1325182_NE_iPSC2.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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