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Sample GSM1325177 Query DataSets for GSM1325177
Status Public on Aug 04, 2014
Title EN_ESC
Sample type RNA
Source name Endodermal cells differentiated from human embryonic stem cells
Organism Homo sapiens
Characteristics cell type: Endodermal cells differentiated from human embryonic stem cells
Extracted molecule total RNA
Extraction protocol Total RNA was isolated by Trizol reagent (Life Technologies) according to the manufacturer's instruction.
Label Cy3
Label protocol Agilent standard protocol
Hybridization protocol Agilent standard protocol
Scan protocol Agilent standard protocol
Description Endodermal cells differentiated from human embryonic stem cells
Data processing The data were analyzed using the Gene Spring GX 11.5.1 software program (Agilent Technologies).The data processing was performed as follows; (i) Threshold raw signals were set to 1.0, (ii) Log base 2 transformation was performed, (iii) 75th percentile normalization was chosen as the normalized algorithm.
Submission date Feb 10, 2014
Last update date Aug 04, 2014
Contact name Kazutoshi Takahashi
Organization name Kyoto University
Department Center for iPS Cell Research and Application
Street address 53 Kawahara-cho, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
Platform ID GPL14550
Series (1)
GSE54848 Critical role of transient activation of human endogenous retroviruses during reprogramming toward pluripotency

Data table header descriptions
VALUE Normalized signal intensity

Data table
(+)E1A_r60_1 10.016586
(+)E1A_r60_3 -6.691024
(+)E1A_r60_a104 -6.5917487
(+)E1A_r60_a107 -2.6862664
(+)E1A_r60_a135 0.5784769
(+)E1A_r60_a20 1.790596
(+)E1A_r60_a22 3.4156857
(+)E1A_r60_a97 5.8358583
(+)E1A_r60_n11 7.6609344
(+)E1A_r60_n9 8.399761
3xSLv1 -6.7081337
A_19_P00315452 0.66594124
A_19_P00315459 -2.0686913
A_19_P00315469 -6.778167
A_19_P00315473 -6.5154104
A_19_P00315482 -5.8146143
A_19_P00315490 -6.6519737
A_19_P00315492 -6.558293
A_19_P00315493 -1.9461813
A_19_P00315496 -6.9543047

Total number of rows: 42545

Table truncated, full table size 1000 Kbytes.

Supplementary file Size Download File type/resource
GSM1325177_EN_ESC.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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