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Sample GSM1325174 Query DataSets for GSM1325174
Status Public on Aug 04, 2014
Title ME_ESC
Sample type RNA
 
Source name Mesendodermal cells differentiated from human embryonic stem cells
Organism Homo sapiens
Characteristics cell type: Mesendodermal cells differentiated from human embryonic stem cells
Extracted molecule total RNA
Extraction protocol Total RNA was isolated by Trizol reagent (Life Technologies) according to the manufacturer's instruction.
Label Cy3
Label protocol Agilent standard protocol
 
Hybridization protocol Agilent standard protocol
Scan protocol Agilent standard protocol
Description Mesendodermal cells differentiated from human embryonic stem cells
Data processing The data were analyzed using the Gene Spring GX 11.5.1 software program (Agilent Technologies).The data processing was performed as follows; (i) Threshold raw signals were set to 1.0, (ii) Log base 2 transformation was performed, (iii) 75th percentile normalization was chosen as the normalized algorithm.
 
Submission date Feb 10, 2014
Last update date Aug 04, 2014
Contact name Kazutoshi Takahashi
E-mail(s) kazu@cira.kyoto-u.ac.jp
Organization name Kyoto University
Department Center for iPS Cell Research and Application
Street address 53 Kawahara-cho, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
 
Platform ID GPL14550
Series (1)
GSE54848 Critical role of transient activation of human endogenous retroviruses during reprogramming toward pluripotency

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
(+)E1A_r60_1 10.130533
(+)E1A_r60_3 -6.888658
(+)E1A_r60_a104 -6.835441
(+)E1A_r60_a107 -2.5794158
(+)E1A_r60_a135 0.7426853
(+)E1A_r60_a20 1.9106874
(+)E1A_r60_a22 3.5572462
(+)E1A_r60_a97 5.944668
(+)E1A_r60_n11 7.8280926
(+)E1A_r60_n9 8.557304
3xSLv1 -6.9501357
A_19_P00315452 -4.067868
A_19_P00315459 -1.2479887
A_19_P00315469 -6.3027296
A_19_P00315473 -6.7650642
A_19_P00315482 -7.0707064
A_19_P00315490 -6.8976583
A_19_P00315492 -6.689166
A_19_P00315493 -1.9711447
A_19_P00315496 -7.2003365

Total number of rows: 42545

Table truncated, full table size 1001 Kbytes.




Supplementary file Size Download File type/resource
GSM1325174_ME_ESC.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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