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Sample GSM1325173 Query DataSets for GSM1325173
Status Public on Aug 04, 2014
Title 253G1-KLF4 dox1 T60
Sample type RNA
Source name KLF4-overexpressed TRA-1-60 (+) human induced pluripotent stem cell line 253G1
Organism Homo sapiens
Characteristics cell type: KLF4-overexpressed human induced pluripotent stem cell line 253G1
Extracted molecule total RNA
Extraction protocol Total RNA was isolated by Trizol reagent (Life Technologies) according to the manufacturer's instruction.
Label Cy3
Label protocol Agilent standard protocol
Hybridization protocol Agilent standard protocol
Scan protocol Agilent standard protocol
Description KLF4-overexpressed human induced pluripotent stem cell line 253G1
Data processing The data were analyzed using the Gene Spring GX 11.5.1 software program (Agilent Technologies).The data processing was performed as follows; (i) Threshold raw signals were set to 1.0, (ii) Log base 2 transformation was performed, (iii) 75th percentile normalization was chosen as the normalized algorithm.
Submission date Feb 10, 2014
Last update date Aug 04, 2014
Contact name Kazutoshi Takahashi
Organization name Kyoto University
Department Center for iPS Cell Research and Application
Street address 53 Kawahara-cho, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
Platform ID GPL14550
Series (1)
GSE54848 Critical role of transient activation of human endogenous retroviruses during reprogramming toward pluripotency

Data table header descriptions
VALUE Normalized signal intensity

Data table
(+)E1A_r60_1 9.472229
(+)E1A_r60_3 -7.225581
(+)E1A_r60_a104 -7.1686053
(+)E1A_r60_a107 -3.2216396
(+)E1A_r60_a135 0.14953804
(+)E1A_r60_a20 1.2570429
(+)E1A_r60_a22 2.9640112
(+)E1A_r60_a97 5.2006674
(+)E1A_r60_n11 7.167696
(+)E1A_r60_n9 7.7978916
3xSLv1 -7.2462974
A_19_P00315452 -1.6674604
A_19_P00315459 -2.19635
A_19_P00315469 -7.1255054
A_19_P00315473 -7.046379
A_19_P00315482 -4.6213655
A_19_P00315490 -7.2786093
A_19_P00315492 -7.366721
A_19_P00315493 -2.7537885
A_19_P00315496 -7.4632025

Total number of rows: 42545

Table truncated, full table size 1001 Kbytes.

Supplementary file Size Download File type/resource
GSM1325173_253G1-KLF4_dox0_T60.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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