|
| Status |
Public on Aug 04, 2014 |
| Title |
KhES3 |
| Sample type |
RNA |
| |
|
| Source name |
human embryonic stem cell line khES3
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human embryonic stem cell line khES3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by Trizol reagent (Life Technologies) according to the manufacturer's instruction.
|
| Label |
Cy3
|
| Label protocol |
Agilent standard protocol
|
| |
|
| Hybridization protocol |
Agilent standard protocol
|
| Scan protocol |
Agilent standard protocol
|
| Description |
human embryonic stem cell line khES3
|
| Data processing |
The data were analyzed using the Gene Spring GX 11.5.1 software program (Agilent Technologies).The data processing was performed as follows; (i) Threshold raw signals were set to 1.0, (ii) Log base 2 transformation was performed, (iii) 75th percentile normalization was chosen as the normalized algorithm.
|
| |
|
| Submission date |
Feb 10, 2014 |
| Last update date |
Aug 04, 2014 |
| Contact name |
Kazutoshi Takahashi |
| E-mail(s) |
kazu@cira.kyoto-u.ac.jp
|
| Organization name |
Kyoto University
|
| Department |
Center for iPS Cell Research and Application
|
| Street address |
53 Kawahara-cho, Shogoin, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL14550 |
| Series (1) |
| GSE54848 |
Critical role of transient activation of human endogenous retroviruses during reprogramming toward pluripotency |
|
