|
Status |
Public on Mar 06, 2014 |
Title |
818THY TALL |
Sample type |
RNA |
|
|
Source name |
Mouse Thymus
|
Organism |
Mus musculus |
Characteristics |
tissue: Thymus disease: T-ALL
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser. Genomic DNA was extracted using standard techniques
|
Label |
Cy3
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays DNA was bisulfite converted, amplified, and labeled
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol DNA was hybridized to the Illumina GoldenGate Cancer Panel I array using the standard Illumina protocol
|
Scan protocol |
Standard Illumina scanning protocol Arrays were imaged using BeadArray Reader using the standard Illumina scanner setting
|
Description |
T-ALL
|
Data processing |
Intensity was generated by the Illumina BeadStudio software and processed by the R/Bioconductor packages Cy3, Cy5, Average Beta, and detection p-values were extracted using BeadStudio software
|
|
|
Submission date |
Feb 07, 2014 |
Last update date |
Mar 06, 2014 |
Contact name |
Yuelin Jack Zhu |
E-mail(s) |
zhujack@mail.nih.gov
|
Phone |
(240)760-7439
|
Organization name |
NCI
|
Department |
Genetics Branch, Center for Cancer Research
|
Lab |
Dr. Paul Meltzer's Lab
|
Street address |
9000 Rockville Pike
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (2) |
GSE54787 |
Gene expression study of leukemic and premalignant hematopoietic tissues from NUP98-PHF23 (NP23) mice. |
GSE54788 |
NUP98-PHF23 |
|