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Sample GSM1323604 Query DataSets for GSM1323604
Status Public on Dec 31, 2014
Title DNA methylation in HMEC
Sample type genomic
 
Source name Cell line
Organism Homo sapiens
Characteristics cell line: HMEC
Growth protocol Du145, PC3, MCF7, MDA-MB-231, and RKO were maintained in RPMI1640 medium. HCT116 was maintained in McCoy's 5A medium. RWPE1 was maintained in keratinocyte-SFM. HMEC was maintained in mammary epithelial cell serum-free growth medium. FHC was maintained in mixture of Ham's F12 and DMEM (1:1).
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted by standard phenol/chloroform method, and was quantified using a Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies).
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
 
Hybridization protocol Standard Illumina Protocol
Scan protocol The array was scanned with an iScan System.
Description Normal cells
Data processing The data were analyzed using GenomeStudio Methylation Module Software.
 
Submission date Feb 06, 2014
Last update date Dec 31, 2014
Contact name Hideyuki Takeshima
E-mail(s) takeshima.hideyuki@hoshi.ac.jp
Organization name Hoshi University
Department Institute for Advanced Life Sciences
Lab Department of Epigenomics
Street address 2-4-41 Ebara
City Shinagawa-ku
State/province Tokyo
ZIP/Postal code 142-8501
Country Japan
 
Platform ID GPL13534
Series (1)
GSE54758 DNA methylation, H3K27me3, and gene expression statuses of human cancer cell lines and normal cells.

Data table header descriptions
ID_REF
VALUE Average Beta
Detection Pval

Data table
ID_REF VALUE Detection Pval
cg00000029 0.06251215 0
cg00000108 0.9387648 0
cg00000109 0.9324962 0
cg00000165 0.144061 0
cg00000236 0.9058168 0
cg00000289 0.7029294 0
cg00000292 0.9117752 0
cg00000321 0.1103577 0
cg00000363 0.1067724 0
cg00000622 0.007045409 0
cg00000658 0.7968856 0
cg00000714 0.1101594 0
cg00000721 0.9365163 0
cg00000734 0.0707368 0
cg00000769 0.0398664 0
cg00000807 0.8478531 0
cg00000884 0.8519958 0
cg00000905 0.07175516 0
cg00000924 0.5932334 0
cg00000948 0.8261989 0

Total number of rows: 485577

Table truncated, full table size 10974 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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