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Sample GSM1323567 Query DataSets for GSM1323567
Status Public on Dec 31, 2014
Title Gene expression in Du145
Sample type RNA
Source name Prostate cancer cell line, Du145
Organism Homo sapiens
Characteristics cell line: Du145
Growth protocol Du145, PC3, MCF7, and MDA-MB-231 were maintained in RPMI1640 medium. RWPE1 was maintained in keratinocyte-SFM. HMEC was maintained in mammary epithelial cell serum-free growth medium.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from cancer cell lines and normal cells using ISOGEN (Nippon Gene, Tokyo, Japan).
Label Cy3
Label protocol From 200 ng of total RNA, Cy3-labeled cRNA was synthesized using Low Input Quick Amp Labeling Kit (Agilent technologies).
Hybridization protocol 600 ng of labeled cRNA was hybridized to SurePrint G3 Human GE v2 8x60K Microarray for 17 hours at 65℃ and 10 rpm rotation. Microarrays was washed according to Agilent protocol.
Scan protocol The microarray was scanned with an Agilent G2565BA microarray scanner (Agilent Technologies).
Description Untreated cells
Data processing Scanned data was analyzed with Feature Extraction Ver.9.1 and analyzed using GeneSpring Ver.12.5 software (Agilent Technologies).
Submission date Feb 06, 2014
Last update date Apr 23, 2018
Contact name Hideyuki Takeshima
Organization name Hoshi University
Department Institute for Advanced Life Sciences
Lab Department of Epigenomics
Street address 2-4-41 Ebara
City Shinagawa-ku
State/province Tokyo
ZIP/Postal code 142-8501
Country Japan
Platform ID GPL17077
Series (1)
GSE54758 DNA methylation, H3K27me3, and gene expression statuses of human cancer cell lines and normal cells.
Reanalyzed by GSE113533

Data table header descriptions
VALUE Normalized signal intensity.

Data table
(+)E1A_r60_1 9.142859
(+)E1A_r60_3 -6.7576094
(+)E1A_r60_a104 -6.58662
(+)E1A_r60_a107 -4.33449
(+)E1A_r60_a135 -1.2556558
(+)E1A_r60_a20 0.25768661
(+)E1A_r60_a22 1.8953066
(+)E1A_r60_a97 4.0022554
(+)E1A_r60_n11 5.9933863
(+)E1A_r60_n9 7.379098
3xSLv1 -6.950867
A_19_P00315452 -0.2643242
A_19_P00315459 -1.9623823
A_19_P00315482 -5.5903845
A_19_P00315492 -5.3436966
A_19_P00315493 -2.4094977
A_19_P00315502 5.180197
A_19_P00315506 -1.3111572
A_19_P00315518 -5.8508797
A_19_P00315519 -6.9462714

Total number of rows: 50739

Table truncated, full table size 1188 Kbytes.

Supplementary file Size Download File type/resource
GSM1323567_Du145_253949414284_1_3.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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