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Sample GSM1322619 Query DataSets for GSM1322619
Status Public on Feb 05, 2014
Title P77S1L14H4: ChronicLocalizedAffected
Sample type RNA
 
Source name periodontal tissue
Organism Homo sapiens
Characteristics patient: 77
specimen: 1
biopsyid: 77.1
diagnosis: Chronic
extent: Localized
condition: Affected
labelbatch: 14
hybridizebatch: 4
Treatment protocol Gingival tissue samples comprising pocket/sulcus epithelium and the underlying connective tissue were obtained from patients with chronic or aggressive periodontitis in conjunction with periodontal surgery
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol Tissue samples were stored in liquid RNA stabilization reagent (RNAlater, Austin, TX) overnight at 4°C, snap-frozen and stored in liquid nitrogen. Specimens were homogenized in Trizol (Invitrogen Life Technologies, Carlsbad, CA), incubated with chloroform and centrifuged at 12,000g. RNA collected in the upper aqueous phase was precipitated by mixing with 75% isopropyl-alcohol and additional centrifugation and washings, and was quantified spectrophotometrically. RNA quality was assessed by determining the RNA Integrity Number (RIN).
Label cyanine 3-pCp
Label protocol miRNA profiling was carried out using the Agilent platform (Agilent Technologies). Total RNA (100 ng/specimen) was labeled to generate fluorescent miRNA by ligation of cyanine 3-pCp to the 3’end
 
Hybridization protocol Labeled RNA hybridized with microarrays carrying probes for 1,205 human and 144 human viral miRNAs (SurePrint G3 Human v16 miRNA 8x60K Microarray Kit).
Scan protocol Array images were scanned with Agilent Technologies Scanner G2505C US83000178 using grid 031181_D_F_20110707 and protocol miRNA_105_Dec08.
Description P77S1L14H4
Data processing The scanned images were analyzed with Feature Extraction Software 10.5.1.1 (Agilent) using 031181_D_F_20110707.xml as design file to obtain background subtracted and Processed Signal intensities. Subsequent data analysis and normalization was performed within R/Bioconductor. Data was normalized and background corrected using rmaMicroRna function with background and normalize set to TRUE. Filtering was performed according to the recommended AgiMicroRna documentation having the following filterMicroRna parameters:
control = TRUE,
IsGeneDetected = TRUE,
wellaboveNEG = FALSE,
limIsGeneDetected = 75,
limNEG = 25
 
Submission date Feb 05, 2014
Last update date Feb 06, 2014
Contact name Paolo Guarnieri
E-mail(s) p.guarnieri@hotmail.com
Organization name Columbia University
Department Systems Biology
Street address 1130 St. Nicholas Avenue
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platform ID GPL15159
Series (1)
GSE54710 MicroRNAs and their target genes in gingival tissues.

Data table header descriptions
ID_REF
VALUE RMA Normalized signal intensity as computed with AgiMicroRna bioconductor package.

Data table
ID_REF VALUE
A_25_P00015345 2.296142726
A_25_P00015343 2.616708724
Blank 1.934448782
dmr_285 9.935658535
dmr_3 14.19015439
dmr_308 2.390013567
dmr_316 2.505566801
dmr_31a 8.944252253
dmr_6 13.13837351
A_25_P00013722 2.365742531
A_25_P00013718 2.348784929
A_25_P00011668 2.945006707
A_25_P00013809 2.37911116
A_25_P00011834 2.392900285
A_25_P00013749 2.301682806
A_25_P00013752 4.562027099
A_25_P00011853 5.5561686
A_25_P00013814 2.302532735
A_25_P00014783 2.331006356
A_25_P00011886 2.302742453

Total number of rows: 1368

Table truncated, full table size 35 Kbytes.




Supplementary file Size Download File type/resource
GSM1322619_US83000178_253118110974_S01_miRNA_105_Dec08_1_2.txt.gz 8.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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