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Sample GSM1322457 Query DataSets for GSM1322457
Status Public on Oct 23, 2014
Title Ro25.6hr.4
Sample type RNA
 
Channel 1
Source name RNA purified from cultured neurons
Organism Mus musculus
Characteristics cell type: neurons
treatment: 1 uM Ro25-4891 for 6 hrs
Growth protocol DIV 21 dissociated mouse hippocampal neurons in 6 well plates were treated by Vehicle, 100 μM AP5, 100 nM NVP-AAM077 or 1 μM Ro25-4891 for 6 hours in a cell culture incubator    
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using RNeasy plus kit (Qiagen)
Label Cy5
Label protocol Briefly, total RNA sample was converted to double-stranded cDNA and then to Cy-dye labeled cRNA using Agilent’s Quick Amp Labeling Kit. The labeled cRNA was purified using RNeasy mini kit (Qiagen, San Diego, CA). cRNA yield and Cy-dye incorporation was determined using ND-1000 spectrophotometer (Thermo Scientific)
 
Channel 2
Source name universal mouse reference (Stratagene, La Jolla, CA)
Organism Mus musculus
Characteristics sample type: reference
Growth protocol DIV 21 dissociated mouse hippocampal neurons in 6 well plates were treated by Vehicle, 100 μM AP5, 100 nM NVP-AAM077 or 1 μM Ro25-4891 for 6 hours in a cell culture incubator    
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using RNeasy plus kit (Qiagen)
Label Cy3
Label protocol Briefly, total RNA sample was converted to double-stranded cDNA and then to Cy-dye labeled cRNA using Agilent’s Quick Amp Labeling Kit. The labeled cRNA was purified using RNeasy mini kit (Qiagen, San Diego, CA). cRNA yield and Cy-dye incorporation was determined using ND-1000 spectrophotometer (Thermo Scientific)
 
 
Hybridization protocol 750 ng of the labeled cRNA was fragmented and hybridized to arrays as described in manufacturer’s hybridization kit
Scan protocol Scanned on an Agilent G2565AA scanner.
Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
Description Ro25.6hr.4
Data processing  Agilent’s Feature Extraction software 11.0 was used to analyze acquired array images
 
Submission date Feb 05, 2014
Last update date Oct 23, 2014
Contact name Josh Kaminker
Organization name Genentech
Department Bioinformatics and Computational Biology
Street address 1 DNA Way
City South San Francisco
State/province CA
ZIP/Postal code 94080
Country USA
 
Platform ID GPL7202
Series (1)
GSE54708 Regulation of neuronal gene expression and survival by basal NMDA receptor activity: a role for Histone Deacetylase 4

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Cy5/Cy3) representing test/reference

Data table
ID_REF VALUE
A_52_P298165 0.385310394
A_52_P96028 0.113089268
A_52_P413492 -2.471691501
A_51_P351598 -0.374995939
A_52_P700056 -2.651842642
A_51_P199778 0.350867703
A_52_P660466 0.025318557
A_52_P62011 0.87794325
A_51_P134184 0.044695808
A_52_P265820 -0.018873494
A_51_P469476 0.169043761
A_52_P61833 0.528973366
A_51_P375631 0.534596835
A_51_P432011 -0.212772459
A_52_P160057 3.375586955
A_52_P633785 0.499883448
A_52_P329881 -0.617838505
A_52_P1053160 -0.525407122
A_52_P677891 3.392131224
A_52_P352541 1.41335066

Total number of rows: 20674

Table truncated, full table size 510 Kbytes.




Supplementary file Size Download File type/resource
GSM1322457_Agilent_251486825547_S01_GE2-v5_95_Feb07_1_4.txt.gz 14.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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