GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1321557 Query DataSets for GSM1321557
Status Public on Feb 04, 2014
Title genomic DNA from Heparin, Fresh Human whole blood 3177
Sample type genomic
Source name Heparin, Fresh Human whole blood 3177HepFr
Organism Homo sapiens
Characteristics sample type: Human whole blood
disease state: normal
sample subtype: Heparin, Fresh
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec; venous whole blood was collected into Heparin
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description X8942297054_R06C02
Infinium Type I and Type II Probes
Heparin, Fresh Human whole blood 3177HepFr
Data processing Unmethylated and methylated signal intensities generated using methylumi in R (see supplementary file on the Series record).
Average Betas (not normalized) generated using GenomeStudio v2010.3
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL13534
Series (2)
GSE54670 Illumina Infinium HumanMethylation450 data for human whole blood samples and for mixtures of purified white blood cells reconstructing human blood
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cg00000029 0.6755437
cg00000108 0.953819
cg00000109 0.9408811
cg00000165 0.3233993
cg00000236 0.8451895
cg00000289 0.7991719
cg00000292 0.8857197
cg00000321 0.4325431
cg00000363 0.486763
cg00000622 0.02733256
cg00000658 0.85864
cg00000714 0.3225287
cg00000721 0.9443561
cg00000734 0.1376931
cg00000769 0.07730447
cg00000807 0.8826097
cg00000884 0.8847089
cg00000905 0.1349256
cg00000924 0.6949233
cg00000948 0.9156132

Total number of rows: 485577

Table truncated, full table size 9998 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap