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Sample GSM1321556 Query DataSets for GSM1321556
Status Public on Feb 04, 2014
Title genomic DNA from Monocytosis Reconstruction mixture
Sample type genomic
Source name Monocytosis Reconstruction mixture Mosis
Organism Homo sapiens
Characteristics sample type: Reconstruction mixture
disease state: normal
sample subtype: Monocytosis
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec; venous whole blood was collected into Heparin
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description X8942297054_R06C01
Infinium Type I and Type II Probes
Monocytosis Reconstruction mixture Mosis
Data processing Unmethylated and methylated signal intensities generated using methylumi in R (see supplementary file on the Series record).
Average Betas (not normalized) generated using GenomeStudio v2010.3
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL13534
Series (2)
GSE54670 Illumina Infinium HumanMethylation450 data for human whole blood samples and for mixtures of purified white blood cells reconstructing human blood
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cg00000029 0.5755854
cg00000108 0.9553649
cg00000109 0.9164981
cg00000165 0.2882712
cg00000236 0.789721
cg00000289 0.7362434
cg00000292 0.8868137
cg00000321 0.332563
cg00000363 0.4485684
cg00000622 0.02261975
cg00000658 0.8664612
cg00000714 0.2689312
cg00000721 0.9373987
cg00000734 0.113837
cg00000769 0.05692573
cg00000807 0.9149559
cg00000884 0.897396
cg00000905 0.1568592
cg00000924 0.6872494
cg00000948 0.9266371

Total number of rows: 485577

Table truncated, full table size 10008 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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