|
Status |
Public on Feb 04, 2014 |
Title |
genomic DNA from Monocytosis Reconstruction mixture |
Sample type |
genomic |
|
|
Source name |
Monocytosis Reconstruction mixture Mosis
|
Organism |
Homo sapiens |
Characteristics |
sample type: Reconstruction mixture disease state: normal sample subtype: Monocytosis
|
Treatment protocol |
Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec; venous whole blood was collected into Heparin
|
Growth protocol |
Not applicable
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450 Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
Description |
X8942297054_R06C01 Infinium Type I and Type II Probes Monocytosis Reconstruction mixture Mosis
|
Data processing |
Unmethylated and methylated signal intensities generated using methylumi in R (see supplementary file on the Series record). Average Betas (not normalized) generated using GenomeStudio v2010.3
|
|
|
Submission date |
Feb 04, 2014 |
Last update date |
Feb 04, 2014 |
Contact name |
William P Accomando |
Organization name |
Harvard School of Public Health
|
Department |
Epidemiology
|
Street address |
Kresge Bldg 911, 677 Huntington Ave
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL13534 |
Series (2) |
GSE54670 |
Illumina Infinium HumanMethylation450 data for human whole blood samples and for mixtures of purified white blood cells reconstructing human blood |
GSE54671 |
Quantitative reconstruction of leukocyte subsets using DNA methylation |
|