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Sample GSM1321555 Query DataSets for GSM1321555
Status Public on Feb 04, 2014
Title genomic DNA from Heparin, Fresh Human whole blood 3159
Sample type genomic
Source name Heparin, Fresh Human whole blood 3159HepFr
Organism Homo sapiens
Characteristics sample type: Human whole blood
disease state: normal
sample subtype: Heparin, Fresh
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec; venous whole blood was collected into Heparin
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description X8942297054_R05C02
Infinium Type I and Type II Probes
Heparin, Fresh Human whole blood 3159HepFr
Data processing Unmethylated and methylated signal intensities generated using methylumi in R (see supplementary file on the Series record).
Average Betas (not normalized) generated using GenomeStudio v2010.3
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL13534
Series (2)
GSE54670 Illumina Infinium HumanMethylation450 data for human whole blood samples and for mixtures of purified white blood cells reconstructing human blood
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cg00000029 0.6143166
cg00000108 0.9515783
cg00000109 0.9028986
cg00000165 0.3110746
cg00000236 0.7911646
cg00000289 0.7816014
cg00000292 0.8741748
cg00000321 0.4339034
cg00000363 0.4609668
cg00000622 0.02372433
cg00000658 0.8621428
cg00000714 0.231296
cg00000721 0.9456422
cg00000734 0.1121029
cg00000769 0.08566722
cg00000807 0.8872783
cg00000884 0.8718158
cg00000905 0.1188282
cg00000924 0.663594
cg00000948 0.9082978

Total number of rows: 485577

Table truncated, full table size 10008 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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