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Sample GSM1321554 Query DataSets for GSM1321554
Status Public on Feb 04, 2014
Title genomic DNA from B-cell Lymphopenia Reconstruction mixture
Sample type genomic
Source name B-cell Lymphopenia Reconstruction mixture Bpen
Organism Homo sapiens
Characteristics sample type: Reconstruction mixture
disease state: normal
sample subtype: B-cell Lymphopenia
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec; venous whole blood was collected into Heparin
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description X8942297054_R05C01
Infinium Type I and Type II Probes
B-cell Lymphopenia Reconstruction mixture Bpen
Data processing Unmethylated and methylated signal intensities generated using methylumi in R (see supplementary file on the Series record).
Average Betas (not normalized) generated using GenomeStudio v2010.3
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL13534
Series (2)
GSE54670 Illumina Infinium HumanMethylation450 data for human whole blood samples and for mixtures of purified white blood cells reconstructing human blood
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cg00000029 0.5748134
cg00000108 0.9572622
cg00000109 0.9143895
cg00000165 0.2910302
cg00000236 0.788439
cg00000289 0.7865348
cg00000292 0.8788863
cg00000321 0.3325367
cg00000363 0.4458432
cg00000622 0.02237315
cg00000658 0.8587195
cg00000714 0.2666667
cg00000721 0.9500544
cg00000734 0.1160151
cg00000769 0.07088627
cg00000807 0.8970107
cg00000884 0.8843587
cg00000905 0.1382694
cg00000924 0.6723892
cg00000948 0.9149771

Total number of rows: 485577

Table truncated, full table size 10010 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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