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Sample GSM1321553 Query DataSets for GSM1321553
Status Public on Feb 04, 2014
Title genomic DNA from Heparin, Fresh Human whole blood 3093
Sample type genomic
Source name Heparin, Fresh Human whole blood 3093HepFr
Organism Homo sapiens
Characteristics sample type: Human whole blood
disease state: normal
sample subtype: Heparin, Fresh
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec; venous whole blood was collected into Heparin
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description X8942297054_R04C02
Infinium Type I and Type II Probes
Heparin, Fresh Human whole blood 3093HepFr
Data processing Unmethylated and methylated signal intensities generated using methylumi in R (see supplementary file on the Series record).
Average Betas (not normalized) generated using GenomeStudio v2010.3
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL13534
Series (2)
GSE54670 Illumina Infinium HumanMethylation450 data for human whole blood samples and for mixtures of purified white blood cells reconstructing human blood
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cg00000029 0.6521989
cg00000108 0.9447646
cg00000109 0.8970497
cg00000165 0.2706271
cg00000236 0.8168852
cg00000289 0.7746409
cg00000292 0.8528507
cg00000321 0.327969
cg00000363 0.4712087
cg00000622 0.03637638
cg00000658 0.8482552
cg00000714 0.2654813
cg00000721 0.9395651
cg00000734 0.1355883
cg00000769 0.08307716
cg00000807 0.8272005
cg00000884 0.8721976
cg00000905 0.1273721
cg00000924 0.6150596
cg00000948 0.8871995

Total number of rows: 485577

Table truncated, full table size 10002 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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