GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1321552 Query DataSets for GSM1321552
Status Public on Feb 04, 2014
Title genomic DNA from Granulocytosis Reconstruction mixture
Sample type genomic
Source name Granulocytosis Reconstruction mixture Gosis
Organism Homo sapiens
Characteristics sample type: Reconstruction mixture
disease state: normal
sample subtype: Granulocytosis
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec; venous whole blood was collected into Heparin
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description X8942297054_R04C01
Infinium Type I and Type II Probes
Granulocytosis Reconstruction mixture Gosis
Data processing Unmethylated and methylated signal intensities generated using methylumi in R (see supplementary file on the Series record).
Average Betas (not normalized) generated using GenomeStudio v2010.3
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL13534
Series (2)
GSE54670 Illumina Infinium HumanMethylation450 data for human whole blood samples and for mixtures of purified white blood cells reconstructing human blood
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cg00000029 0.572803
cg00000108 0.9534308
cg00000109 0.910888
cg00000165 0.3359618
cg00000236 0.7443891
cg00000289 0.7553838
cg00000292 0.8775734
cg00000321 0.3118434
cg00000363 0.4315983
cg00000622 0.03125
cg00000658 0.8432081
cg00000714 0.2630413
cg00000721 0.9523215
cg00000734 0.1310801
cg00000769 0.07448988
cg00000807 0.8999893
cg00000884 0.8777648
cg00000905 0.1479601
cg00000924 0.652365
cg00000948 0.9102761

Total number of rows: 485577

Table truncated, full table size 10008 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap