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Sample GSM1321551 Query DataSets for GSM1321551
Status Public on Feb 04, 2014
Title genomic DNA from Heparin, Fresh Human whole blood 3082
Sample type genomic
Source name Heparin, Fresh Human whole blood 3082HepFr
Organism Homo sapiens
Characteristics sample type: Human whole blood
disease state: normal
sample subtype: Heparin, Fresh
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec; venous whole blood was collected into Heparin
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description X8942297054_R03C02
Infinium Type I and Type II Probes
Heparin, Fresh Human whole blood 3082HepFr
Data processing Unmethylated and methylated signal intensities generated using methylumi in R (see supplementary file on the Series record).
Average Betas (not normalized) generated using GenomeStudio v2010.3
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL13534
Series (2)
GSE54670 Illumina Infinium HumanMethylation450 data for human whole blood samples and for mixtures of purified white blood cells reconstructing human blood
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cg00000029 0.5496395
cg00000108 0.9319506
cg00000109 0.8730884
cg00000165 0.3224727
cg00000236 0.7883212
cg00000289 0.7464356
cg00000292 0.8375021
cg00000321 0.3102115
cg00000363 0.4768928
cg00000622 0.03308982
cg00000658 0.8270966
cg00000714 0.2736783
cg00000721 0.9166308
cg00000734 0.1247961
cg00000769 0.07749368
cg00000807 0.8368569
cg00000884 0.8562697
cg00000905 0.1436376
cg00000924 0.634737
cg00000948 0.8668609

Total number of rows: 485577

Table truncated, full table size 9995 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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