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Sample GSM1321550 Query DataSets for GSM1321550
Status Public on Feb 04, 2014
Title genomic DNA from Granulocytopenia Reconstruction mixture
Sample type genomic
Source name Granulocytopenia Reconstruction mixture Gpen
Organism Homo sapiens
Characteristics sample type: Reconstruction mixture
disease state: normal
sample subtype: Granulocytopenia
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec; venous whole blood was collected into Heparin
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description X8942297054_R03C01
Infinium Type I and Type II Probes
Granulocytopenia Reconstruction mixture Gpen
Data processing Unmethylated and methylated signal intensities generated using methylumi in R (see supplementary file on the Series record).
Average Betas (not normalized) generated using GenomeStudio v2010.3
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL13534
Series (2)
GSE54670 Illumina Infinium HumanMethylation450 data for human whole blood samples and for mixtures of purified white blood cells reconstructing human blood
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cg00000029 0.579416
cg00000108 0.9332789
cg00000109 0.8676611
cg00000165 0.2366534
cg00000236 0.8080238
cg00000289 0.7874187
cg00000292 0.8436956
cg00000321 0.3605331
cg00000363 0.4187472
cg00000622 0.04131929
cg00000658 0.7846809
cg00000714 0.2815049
cg00000721 0.9300275
cg00000734 0.08922352
cg00000769 0.09292708
cg00000807 0.836351
cg00000884 0.8484366
cg00000905 0.1349734
cg00000924 0.5654772
cg00000948 0.8907192

Total number of rows: 485577

Table truncated, full table size 9998 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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