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Sample GSM1321547 Query DataSets for GSM1321547
Status Public on Feb 04, 2014
Title genomic DNA from Heparin, Fresh Human whole blood 3021
Sample type genomic
Source name Heparin, Fresh Human whole blood 3021HepFr
Organism Homo sapiens
Characteristics sample type: Human whole blood
disease state: normal
sample subtype: Heparin, Fresh
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec; venous whole blood was collected into Heparin
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description X8942297054_R01C02
Infinium Type I and Type II Probes
Heparin, Fresh Human whole blood 3021HepFr
Data processing Unmethylated and methylated signal intensities generated using methylumi in R (see supplementary file on the Series record).
Average Betas (not normalized) generated using GenomeStudio v2010.3
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL13534
Series (2)
GSE54670 Illumina Infinium HumanMethylation450 data for human whole blood samples and for mixtures of purified white blood cells reconstructing human blood
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cg00000029 0.5784553
cg00000108 0.9243139
cg00000109 0.8604167
cg00000165 0.3037397
cg00000236 0.7720238
cg00000289 0.7580478
cg00000292 0.8744366
cg00000321 0.3167902
cg00000363 0.4903414
cg00000622 0.02911502
cg00000658 0.8202899
cg00000714 0.2931342
cg00000721 0.8821737
cg00000734 0.1175426
cg00000769 0.07382265
cg00000807 0.821292
cg00000884 0.7676887
cg00000905 0.1461809
cg00000924 0.7104573
cg00000948 0.8979377

Total number of rows: 485577

Table truncated, full table size 9974 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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