GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1321546 Query DataSets for GSM1321546
Status Public on Feb 04, 2014
Title genomic DNA from Normal Reconstruction mixture Reconstruction mixture
Sample type genomic
Source name Normal Reconstruction mixture Reconstruction mixture NormMix
Organism Homo sapiens
Characteristics sample type: Reconstruction mixture
disease state: normal
sample subtype: Normal Reconstruction mixture
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec; venous whole blood was collected into Heparin
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description X8942297054_R01C01
Infinium Type I and Type II Probes
Normal Reconstruction mixture Reconstruction mixture NormMix
Data processing Unmethylated and methylated signal intensities generated using methylumi in R (see supplementary file on the Series record).
Average Betas (not normalized) generated using GenomeStudio v2010.3
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL13534
Series (2)
GSE54670 Illumina Infinium HumanMethylation450 data for human whole blood samples and for mixtures of purified white blood cells reconstructing human blood
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cg00000029 0.5062945
cg00000108 0.9246999
cg00000109 0.895792
cg00000165 0.2153523
cg00000236 0.7572414
cg00000289 0.7027601
cg00000292 0.8196359
cg00000321 0.3115169
cg00000363 0.3995938
cg00000622 0.02474876
cg00000658 0.7966746
cg00000714 0.2361035
cg00000721 0.8990428
cg00000734 0.09982122
cg00000769 0.08873353
cg00000807 0.8303953
cg00000884 0.8452013
cg00000905 0.07697513
cg00000924 0.5811673
cg00000948 0.8532274

Total number of rows: 485577

Table truncated, full table size 10018 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap