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Sample GSM1320797 Query DataSets for GSM1320797
Status Public on Feb 04, 2014
Title genomic DNA from Tregs Purified WBC subtype (Treg2280)
Sample type genomic
Source name Tregs Purified WBC subtype Treg2280
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: Tregs
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Treg2280
Tregs Purified WBC subtype Treg2280
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.0406293
foxp3DMR1in 0.6671574
foxp3DMRpro 0.3651468
cg00071250 0.03105151
cg00226923 0.9653823
cg00431549 0.1227708
cg00491404 0.6156527
cg00546897 0.9548038
cg00554250 0.3253302
cg00620024 0.095171
cg00673191 0.8111812
cg00739120 0.5726604
cg00795812 0.05691429
cg00899659 0.9247341
cg01031251 0.9616522
cg01124420 0.1474758
cg01182697 0.6818545
cg01278291 0.7307792
cg01344171 0.536933
cg01441777 0.8866516

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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