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Sample GSM1320777 Query DataSets for GSM1320777
Status Public on Feb 04, 2014
Title genomic DNA from Basophils Purified WBC subtype (Baso2492)
Sample type genomic
Source name Basophils Purified WBC subtype Baso2492
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: Basophils
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Baso2492
Basophils Purified WBC subtype Baso2492
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.9888693
foxp3DMR1in 0.9765153
foxp3DMRpro 0.925544
cg00071250 0.9221449
cg00226923 0.9806631
cg00431549 0.02678112
cg00491404 0.8674906
cg00546897 0.9562118
cg00554250 0.07283626
cg00620024 0.9402955
cg00673191 0.4571546
cg00739120 0.08111834
cg00795812 0.9785105
cg00899659 0.0402331
cg01031251 0.9438648
cg01124420 0.8256659
cg01182697 0.05115935
cg01278291 0.4951472
cg01344171 0.5169673
cg01441777 0.3118213

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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