NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1320775 Query DataSets for GSM1320775
Status Public on Feb 04, 2014
Title genomic DNA from Granulocytes Purified WBC subtype (Gran1150)
Sample type genomic
 
Source name Granulocytes Purified WBC subtype Gran1150
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: Granulocytes
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
 
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Gran1150
Granulocytes Purified WBC subtype Gran1150
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
 
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
ID_REF
VALUE Average Beta (0-1 constrained), not normalized

Data table
ID_REF VALUE
cd3zDMR 0.9898701
foxp3DMR1in 0.983631
foxp3DMRpro 0.965311
cg00071250 0.9356912
cg00226923 0.9805692
cg00431549 0.02444386
cg00491404 0.8811534
cg00546897 0.4979846
cg00554250 0.07303684
cg00620024 0.9090266
cg00673191 0.2924379
cg00739120 0.1674983
cg00795812 0.9851989
cg00899659 0.02123218
cg01031251 0.8639545
cg01124420 0.8026409
cg01182697 0.0341641
cg01278291 0.1672629
cg01344171 0.6366571
cg01441777 0.423454

Total number of rows: 96

Table truncated, full table size 1 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap