|
Status |
Public on Feb 04, 2014 |
Title |
genomic DNA from Granulocytes Purified WBC subtype (Gran1150) |
Sample type |
genomic |
|
|
Source name |
Granulocytes Purified WBC subtype Gran1150
|
Organism |
Homo sapiens |
Characteristics |
sample type: Purified WBC subtype disease state: normal sample subtype: Granulocytes
|
Treatment protocol |
Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
|
Growth protocol |
Not applicable
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
Description |
Gran1150 Granulocytes Purified WBC subtype Gran1150 Illumina VeraCode GGMA
|
Data processing |
Illumina GenomeStudio v2010.3 Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
|
|
|
Submission date |
Feb 04, 2014 |
Last update date |
Feb 04, 2014 |
Contact name |
William P Accomando |
Organization name |
Harvard School of Public Health
|
Department |
Epidemiology
|
Street address |
Kresge Bldg 911, 677 Huntington Ave
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL18246 |
Series (2) |
GSE54647 |
VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples |
GSE54671 |
Quantitative reconstruction of leukocyte subsets using DNA methylation |
|