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Sample GSM1320774 Query DataSets for GSM1320774
Status Public on Feb 04, 2014
Title genomic DNA from Pan-NK cells Purified WBC subtype (NKpan2510)
Sample type genomic
Source name Pan-NK cells Purified WBC subtype NKpan2510
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: Pan-NK cells
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description NKpan2510
Pan-NK cells Purified WBC subtype NKpan2510
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.1670275
foxp3DMR1in 0.9742018
foxp3DMRpro 0.8416119
cg00071250 0.02173139
cg00226923 0.9696563
cg00431549 0.02379849
cg00491404 0.1874146
cg00546897 0.9667691
cg00554250 0.1194022
cg00620024 0.840196
cg00673191 0.7963042
cg00739120 0.2352271
cg00795812 0.8599791
cg00899659 0.4331063
cg01031251 0.9677229
cg01124420 0.5452737
cg01182697 0.1904592
cg01278291 0.7208039
cg01344171 0.6626904
cg01441777 0.5276591

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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