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Sample GSM1320770 Query DataSets for GSM1320770
Status Public on Feb 04, 2014
Title genomic DNA from Monocytes Purified WBC subtype (Mono1993)
Sample type genomic
Source name Monocytes Purified WBC subtype Mono1993
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: Monocytes
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Mono1993
Monocytes Purified WBC subtype Mono1993
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.9884187
foxp3DMR1in 0.9777479
foxp3DMRpro 0.9709072
cg00071250 0.9009258
cg00226923 0.9782068
cg00431549 0.02341877
cg00491404 0.854705
cg00546897 0.2218071
cg00554250 0.08724305
cg00620024 0.9319746
cg00673191 0.7471657
cg00739120 0.1183286
cg00795812 0.8532975
cg00899659 0.05291033
cg01031251 0.95293
cg01124420 0.7819037
cg01182697 0.0521584
cg01278291 0.7513532
cg01344171 0.6425741
cg01441777 0.3848725

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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