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Sample GSM1320768 Query DataSets for GSM1320768
Status Public on Feb 04, 2014
Title genomic DNA from CD8+ T-cells Purified WBC subtype (Tc2007)
Sample type genomic
Source name CD8+ T-cells Purified WBC subtype Tc2007
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: CD8+ T-cells
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Tc2007
CD8+ T-cells Purified WBC subtype Tc2007
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.06331319
foxp3DMR1in 0.9593807
foxp3DMRpro 0.4025576
cg00071250 0.02515266
cg00226923 0.9651887
cg00431549 0.05419996
cg00491404 0.4365586
cg00546897 0.9609266
cg00554250 0.1664783
cg00620024 0.219033
cg00673191 0.7384096
cg00739120 0.6448945
cg00795812 0.07333221
cg00899659 0.7774353
cg01031251 0.9584015
cg01124420 0.2534436
cg01182697 0.8592036
cg01278291 0.892969
cg01344171 0.8780846
cg01441777 0.9416256

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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