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Sample GSM1320765 Query DataSets for GSM1320765
Status Public on Feb 04, 2014
Title genomic DNA from CD8- NK cells Purified WBC subtype (NK8m2631)
Sample type genomic
Source name CD8- NK cells Purified WBC subtype NK8m2631
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: CD8- NK cells
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description NK8m2631
CD8- NK cells Purified WBC subtype NK8m2631
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.3260131
foxp3DMR1in 0.9668401
foxp3DMRpro 0.8793864
cg00071250 0.04274008
cg00226923 0.9573253
cg00431549 0.02248092
cg00491404 0.2171223
cg00546897 0.9235844
cg00554250 0.1436045
cg00620024 0.8668158
cg00673191 0.7945043
cg00739120 0.2085138
cg00795812 0.8363071
cg00899659 0.5493845
cg01031251 0.9640227
cg01124420 0.6033771
cg01182697 0.2081976
cg01278291 0.7399486
cg01344171 0.3607194
cg01441777 0.6036111

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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