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Sample GSM1320764 Query DataSets for GSM1320764
Status Public on Feb 04, 2014
Title genomic DNA from Granulocytes Purified WBC subtype (Gran1149)
Sample type genomic
Source name Granulocytes Purified WBC subtype Gran1149
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: Granulocytes
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Gran1149
Granulocytes Purified WBC subtype Gran1149
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.9894745
foxp3DMR1in 0.9831352
foxp3DMRpro 0.9682088
cg00071250 0.9486104
cg00226923 0.9795183
cg00431549 0.0302361
cg00491404 0.8946742
cg00546897 0.6376861
cg00554250 0.06061746
cg00620024 0.9234666
cg00673191 0.2544847
cg00739120 0.2066628
cg00795812 0.984906
cg00899659 0.02058571
cg01031251 0.8359776
cg01124420 0.8125356
cg01182697 0.03652543
cg01278291 0.09810529
cg01344171 0.5999559
cg01441777 0.4444035

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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