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Sample GSM1320758 Query DataSets for GSM1320758
Status Public on Feb 04, 2014
Title genomic DNA from NKT cells Purified WBC subtype (NKT2616)
Sample type genomic
Source name NKT cells Purified WBC subtype NKT2616
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: NKT cells
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description NKT2616
NKT cells Purified WBC subtype NKT2616
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.06000702
foxp3DMR1in 0.9141284
foxp3DMRpro 0.7887095
cg00071250 0.02044587
cg00226923 0.9559308
cg00431549 0.03553232
cg00491404 0.3824868
cg00546897 0.9670653
cg00554250 0.2778501
cg00620024 0.2161201
cg00673191 0.7276117
cg00739120 0.6374012
cg00795812 0.1960138
cg00899659 0.8976065
cg01031251 0.9581017
cg01124420 0.06681634
cg01182697 0.8120952
cg01278291 0.8951588
cg01344171 0.8716648
cg01441777 0.9504864

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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